Abstract
A number of studies have documented suppression of lymphocyte activation in mice infected with Salmonella. Here, we describe incomplete activation of CD4+ T cells following intravenous injection of specific peptide and LPS into Salmonella-infected mice. Although antigen-specific CD4+ T cells were activated by peptide/LPS to increase surface CD69 expression, they did not produce IL-2 or TNF-α. Suppression of cytokine production did not require prolonged exposure of the T cells to the Salmonella-infected environment, was not antigen specific, but was dependent upon the presence of LPS during stimulation. These data suggest that Salmonella-infected mice are exquisitely sensitive to the generation of a suppressive environment following innate immune stimulation with LPS. In agreement with this interpretation, repeated low-dose administration of LPS caused uncontrolled replication of attenuated Salmonella in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 403-411 |
| Number of pages | 9 |
| Journal | Journal of Leukocyte Biology |
| Volume | 81 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2007 |
| Externally published | Yes |
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Keywords
- Bacterial infection
- IL-2
- Tumor necrosis factor-α
ASJC Scopus subject areas
- Cell Biology
Cite this
Pivotal Advance : Exposure to LPS suppresses CD4+ T cell cytokine production in Salmonella-infected mice and exacerbates murine typhoid. / Srinivasan, Aparna; Mcsorley, Stephen J.
In: Journal of Leukocyte Biology, Vol. 81, No. 2, 01.02.2007, p. 403-411.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pivotal Advance
T2 - Exposure to LPS suppresses CD4+ T cell cytokine production in Salmonella-infected mice and exacerbates murine typhoid
AU - Srinivasan, Aparna
AU - Mcsorley, Stephen J
PY - 2007/2/1
Y1 - 2007/2/1
N2 - A number of studies have documented suppression of lymphocyte activation in mice infected with Salmonella. Here, we describe incomplete activation of CD4+ T cells following intravenous injection of specific peptide and LPS into Salmonella-infected mice. Although antigen-specific CD4+ T cells were activated by peptide/LPS to increase surface CD69 expression, they did not produce IL-2 or TNF-α. Suppression of cytokine production did not require prolonged exposure of the T cells to the Salmonella-infected environment, was not antigen specific, but was dependent upon the presence of LPS during stimulation. These data suggest that Salmonella-infected mice are exquisitely sensitive to the generation of a suppressive environment following innate immune stimulation with LPS. In agreement with this interpretation, repeated low-dose administration of LPS caused uncontrolled replication of attenuated Salmonella in vivo.
AB - A number of studies have documented suppression of lymphocyte activation in mice infected with Salmonella. Here, we describe incomplete activation of CD4+ T cells following intravenous injection of specific peptide and LPS into Salmonella-infected mice. Although antigen-specific CD4+ T cells were activated by peptide/LPS to increase surface CD69 expression, they did not produce IL-2 or TNF-α. Suppression of cytokine production did not require prolonged exposure of the T cells to the Salmonella-infected environment, was not antigen specific, but was dependent upon the presence of LPS during stimulation. These data suggest that Salmonella-infected mice are exquisitely sensitive to the generation of a suppressive environment following innate immune stimulation with LPS. In agreement with this interpretation, repeated low-dose administration of LPS caused uncontrolled replication of attenuated Salmonella in vivo.
KW - Bacterial infection
KW - IL-2
KW - Tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=33846695774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846695774&partnerID=8YFLogxK
U2 - 10.1189/jlb.0306194
DO - 10.1189/jlb.0306194
M3 - Article
C2 - 16916961
AN - SCOPUS:33846695774
VL - 81
SP - 403
EP - 411
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 2
ER -