Pivotal Advance

Exposure to LPS suppresses CD4+ T cell cytokine production in Salmonella-infected mice and exacerbates murine typhoid

Aparna Srinivasan, Stephen J Mcsorley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A number of studies have documented suppression of lymphocyte activation in mice infected with Salmonella. Here, we describe incomplete activation of CD4+ T cells following intravenous injection of specific peptide and LPS into Salmonella-infected mice. Although antigen-specific CD4+ T cells were activated by peptide/LPS to increase surface CD69 expression, they did not produce IL-2 or TNF-α. Suppression of cytokine production did not require prolonged exposure of the T cells to the Salmonella-infected environment, was not antigen specific, but was dependent upon the presence of LPS during stimulation. These data suggest that Salmonella-infected mice are exquisitely sensitive to the generation of a suppressive environment following innate immune stimulation with LPS. In agreement with this interpretation, repeated low-dose administration of LPS caused uncontrolled replication of attenuated Salmonella in vivo.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalJournal of Leukocyte Biology
Volume81
Issue number2
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Typhoid Fever
Salmonella
Cytokines
T-Lymphocytes
CD4 Antigens
Peptides
Lymphocyte Activation
Intravenous Injections
Interleukin-2
Antigens

Keywords

  • Bacterial infection
  • IL-2
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Cell Biology

Cite this

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abstract = "A number of studies have documented suppression of lymphocyte activation in mice infected with Salmonella. Here, we describe incomplete activation of CD4+ T cells following intravenous injection of specific peptide and LPS into Salmonella-infected mice. Although antigen-specific CD4+ T cells were activated by peptide/LPS to increase surface CD69 expression, they did not produce IL-2 or TNF-α. Suppression of cytokine production did not require prolonged exposure of the T cells to the Salmonella-infected environment, was not antigen specific, but was dependent upon the presence of LPS during stimulation. These data suggest that Salmonella-infected mice are exquisitely sensitive to the generation of a suppressive environment following innate immune stimulation with LPS. In agreement with this interpretation, repeated low-dose administration of LPS caused uncontrolled replication of attenuated Salmonella in vivo.",
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