Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications

P. Lange, K. Greco, L. Partington, C. Carvalho, S. Oliani, M. A. Birchall, P. D. Sibbons, M. W. Lowdell, T. Ansari

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum technology, which when combined with an enzyme/detergent protocol significantly reduces the time required to create clinically suitable scaffolds. We have applied this technology to prepare porcine tracheal scaffolds and compared the results to scaffolds produced under normal atmospheric pressures. The principal outcome measures were the reduction in time (9 days to prepare the scaffold) followed by a reduction in residual DNA levels (DC no-vac: 137.8±48.82 ng/mg vs. DC vac 36.83±18.45 ng/mg, p<0.05.). Our approach did not impact on the collagen or glycosaminoglycan content or on the biomechanical properties of the scaffolds. We applied the vacuum technology to human tracheae, which, when implanted in vivo showed no significant adverse immunological response. The addition of a vacuum to a conventional decellularization protocol significantly reduces production time, whilst providing a suitable scaffold. This increases clinical utility and lowers production costs. To our knowledge this is the first time that vacuum assisted decellularization has been explored.

Original languageEnglish (US)
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
StateAccepted/In press - 2015
Externally publishedYes

Fingerprint

Biomedical Engineering
Tissue Engineering
Vacuum
Trachea
Tissue engineering
Scaffolds
Technology
Vacuum technology
Costs and Cost Analysis
Atmospheric Pressure
Glycosaminoglycans
Detergents
Swine
Collagen
Outcome Assessment (Health Care)
DNA
Enzymes
Atmospheric pressure
Costs
Tissue

Keywords

  • Decellularization
  • Tissue engineering
  • Trachea
  • Transplantation
  • Vacuum

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications. / Lange, P.; Greco, K.; Partington, L.; Carvalho, C.; Oliani, S.; Birchall, M. A.; Sibbons, P. D.; Lowdell, M. W.; Ansari, T.

In: Journal of Tissue Engineering and Regenerative Medicine, 2015.

Research output: Contribution to journalArticle

Lange, P. ; Greco, K. ; Partington, L. ; Carvalho, C. ; Oliani, S. ; Birchall, M. A. ; Sibbons, P. D. ; Lowdell, M. W. ; Ansari, T. / Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications. In: Journal of Tissue Engineering and Regenerative Medicine. 2015.
@article{c2cd977fc8fa48828e58b015d065d43e,
title = "Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications",
abstract = "Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum technology, which when combined with an enzyme/detergent protocol significantly reduces the time required to create clinically suitable scaffolds. We have applied this technology to prepare porcine tracheal scaffolds and compared the results to scaffolds produced under normal atmospheric pressures. The principal outcome measures were the reduction in time (9 days to prepare the scaffold) followed by a reduction in residual DNA levels (DC no-vac: 137.8±48.82 ng/mg vs. DC vac 36.83±18.45 ng/mg, p<0.05.). Our approach did not impact on the collagen or glycosaminoglycan content or on the biomechanical properties of the scaffolds. We applied the vacuum technology to human tracheae, which, when implanted in vivo showed no significant adverse immunological response. The addition of a vacuum to a conventional decellularization protocol significantly reduces production time, whilst providing a suitable scaffold. This increases clinical utility and lowers production costs. To our knowledge this is the first time that vacuum assisted decellularization has been explored.",
keywords = "Decellularization, Tissue engineering, Trachea, Transplantation, Vacuum",
author = "P. Lange and K. Greco and L. Partington and C. Carvalho and S. Oliani and Birchall, {M. A.} and Sibbons, {P. D.} and Lowdell, {M. W.} and T. Ansari",
year = "2015",
doi = "10.1002/term.1979",
language = "English (US)",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications

AU - Lange, P.

AU - Greco, K.

AU - Partington, L.

AU - Carvalho, C.

AU - Oliani, S.

AU - Birchall, M. A.

AU - Sibbons, P. D.

AU - Lowdell, M. W.

AU - Ansari, T.

PY - 2015

Y1 - 2015

N2 - Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum technology, which when combined with an enzyme/detergent protocol significantly reduces the time required to create clinically suitable scaffolds. We have applied this technology to prepare porcine tracheal scaffolds and compared the results to scaffolds produced under normal atmospheric pressures. The principal outcome measures were the reduction in time (9 days to prepare the scaffold) followed by a reduction in residual DNA levels (DC no-vac: 137.8±48.82 ng/mg vs. DC vac 36.83±18.45 ng/mg, p<0.05.). Our approach did not impact on the collagen or glycosaminoglycan content or on the biomechanical properties of the scaffolds. We applied the vacuum technology to human tracheae, which, when implanted in vivo showed no significant adverse immunological response. The addition of a vacuum to a conventional decellularization protocol significantly reduces production time, whilst providing a suitable scaffold. This increases clinical utility and lowers production costs. To our knowledge this is the first time that vacuum assisted decellularization has been explored.

AB - Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum technology, which when combined with an enzyme/detergent protocol significantly reduces the time required to create clinically suitable scaffolds. We have applied this technology to prepare porcine tracheal scaffolds and compared the results to scaffolds produced under normal atmospheric pressures. The principal outcome measures were the reduction in time (9 days to prepare the scaffold) followed by a reduction in residual DNA levels (DC no-vac: 137.8±48.82 ng/mg vs. DC vac 36.83±18.45 ng/mg, p<0.05.). Our approach did not impact on the collagen or glycosaminoglycan content or on the biomechanical properties of the scaffolds. We applied the vacuum technology to human tracheae, which, when implanted in vivo showed no significant adverse immunological response. The addition of a vacuum to a conventional decellularization protocol significantly reduces production time, whilst providing a suitable scaffold. This increases clinical utility and lowers production costs. To our knowledge this is the first time that vacuum assisted decellularization has been explored.

KW - Decellularization

KW - Tissue engineering

KW - Trachea

KW - Transplantation

KW - Vacuum

UR - http://www.scopus.com/inward/record.url?scp=84923266245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923266245&partnerID=8YFLogxK

U2 - 10.1002/term.1979

DO - 10.1002/term.1979

M3 - Article

C2 - 25689270

AN - SCOPUS:84923266245

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

ER -