PIK3CAH1047R induces multipotency and multi-lineage mammary tumours

Shany Koren, Linsey Reavie, Joana Pinto Couto, Duvini De Silva, Michael B. Stadler, Tim Roloff, Adrian Britschgi, Tobias Eichlisberger, Hubertus Kohler, Olulanu Aina, Robert Cardiff, Mohamed Bentires-Alj

Research output: Contribution to journalArticle

139 Scopus citations

Abstract

The adult mouse mammary epithelium contains self-sustained cell lineages that form the inner luminal and outer basal cell layers, with stem and progenitor cells contributing to its proliferative and regenerative potential. A key issue in breast cancer biology is the effect of genomic lesions in specific mammary cell lineages on tumour heterogeneity and progression. The impact of transforming events on fate conversion in cancer cells of origin and thus their contribution to tumour heterogeneity remains largely elusive. Using in situ genetic lineage tracing and limiting dilution transplantation, we have unravelled the potential of PIK3CAH1047R, one of the most frequent mutations occurring in human breast cancer, to induce multipotency during tumorigenesis in the mammary gland. Here we show that expression of PIK3CAH1047R in lineage-committed basal Lgr5-positive and luminal keratin-8-positive cells of the adult mouse mammary gland evokes cell dedifferentiation into a multipotent stem-like state, suggesting this to be a mechanism involved in the formation of heterogeneous, multi-lineage mammary tumours. Moreover, we show that the tumour cell of origin influences the frequency of malignant mammary tumours. Our results define a key effect of PIK3CAH1047R on mammary cell fate in the pre-neoplastic mammary gland and show that the cell of origin of PIK3CAH1047R tumours dictates their malignancy, thus revealing a mechanism underlying tumour heterogeneity and aggressiveness.

Original languageEnglish (US)
Pages (from-to)114-118
Number of pages5
JournalNature
Volume525
Issue number7567
DOIs
StatePublished - Sep 3 2015

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    Koren, S., Reavie, L., Couto, J. P., De Silva, D., Stadler, M. B., Roloff, T., Britschgi, A., Eichlisberger, T., Kohler, H., Aina, O., Cardiff, R., & Bentires-Alj, M. (2015). PIK3CAH1047R induces multipotency and multi-lineage mammary tumours. Nature, 525(7567), 114-118. https://doi.org/10.1038/nature14669