Pif1 family helicases suppress genome instability at G-quadruplex motifs

Katrin Paeschke; Matthew L. Bochman; P. Daniela Garcia; Petr Cejka; Katherine L. Friedman; Stephen C. Kowalczykowski; Virginia A. Zakian

doi:10.1038/nature12149

Pif1 family helicases suppress genome instability at G-quadruplex motifs

Katrin Paeschke, Matthew L. Bochman, P. Daniela Garcia, Petr Cejka, Katherine L. Friedman, Stephen C. Kowalczykowski, Virginia A. Zakian

Microbiology

Research output: Contribution to journal › Article › peer-review

295 Scopus citations

Abstract

The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

Original language	English (US)
Pages (from-to)	458-462
Number of pages	5
Journal	Nature
Volume	497
Issue number	7450
DOIs	https://doi.org/10.1038/nature12149
State	Published - 2013

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title = "Pif1 family helicases suppress genome instability at G-quadruplex motifs",

abstract = "The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.",

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T1 - Pif1 family helicases suppress genome instability at G-quadruplex motifs

AU - Paeschke, Katrin

AU - Bochman, Matthew L.

AU - Daniela Garcia, P.

AU - Cejka, Petr

AU - Friedman, Katherine L.

AU - Kowalczykowski, Stephen C.

AU - Zakian, Virginia A.

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AB - The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

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Pif1 family helicases suppress genome instability at G-quadruplex motifs

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