Pif1 family helicases suppress genome instability at G-quadruplex motifs

Katrin Paeschke; Matthew L. Bochman; P. Daniela Garcia; Petr Cejka; Katherine L. Friedman; Stephen C. Kowalczykowski; Virginia A. Zakian

doi:10.1038/nature12149

Pif1 family helicases suppress genome instability at G-quadruplex motifs

Katrin Paeschke, Matthew L. Bochman, P. Daniela Garcia, Petr Cejka, Katherine L. Friedman, Stephen C. Kowalczykowski, Virginia A. Zakian

Microbiology

Research output: Contribution to journal › Article

240 Scopus citations

Abstract

The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

Original language	English (US)
Pages (from-to)	458-462
Number of pages	5
Journal	Nature
Volume	497
Issue number	7450
DOIs	https://doi.org/10.1038/nature12149
State	Published - 2013

ASJC Scopus subject areas

General

Access to Document

10.1038/nature12149

Fingerprint Dive into the research topics of 'Pif1 family helicases suppress genome instability at G-quadruplex motifs'. Together they form a unique fingerprint.

Cite this

Paeschke, K., Bochman, M. L., Daniela Garcia, P., Cejka, P., Friedman, K. L., Kowalczykowski, S. C., & Zakian, V. A. (2013). Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature, 497(7450), 458-462. https://doi.org/10.1038/nature12149

@article{0d0b228f286f4031b3a431bb0e06b6f2,

title = "Pif1 family helicases suppress genome instability at G-quadruplex motifs",

abstract = "The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.",

author = "Katrin Paeschke and Bochman, {Matthew L.} and {Daniela Garcia}, P. and Petr Cejka and Friedman, {Katherine L.} and Kowalczykowski, {Stephen C.} and Zakian, {Virginia A.}",

year = "2013",

doi = "10.1038/nature12149",

language = "English (US)",

volume = "497",

pages = "458--462",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "7450",

}

TY - JOUR

T1 - Pif1 family helicases suppress genome instability at G-quadruplex motifs

AU - Paeschke, Katrin

AU - Bochman, Matthew L.

AU - Daniela Garcia, P.

AU - Cejka, Petr

AU - Friedman, Katherine L.

AU - Kowalczykowski, Stephen C.

AU - Zakian, Virginia A.

PY - 2013

Y1 - 2013

N2 - The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

AB - The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

UR - http://www.scopus.com/inward/record.url?scp=84878122437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878122437&partnerID=8YFLogxK

U2 - 10.1038/nature12149

DO - 10.1038/nature12149

M3 - Article

C2 - 23657261

AN - SCOPUS:84878122437

VL - 497

SP - 458

EP - 462

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7450

ER -