Abstract
The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 458-462 |
| Number of pages | 5 |
| Journal | Nature |
| Volume | 497 |
| Issue number | 7450 |
| DOIs | |
| State | Published - 2013 |
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Pif1 family helicases suppress genome instability at G-quadruplex motifs. / Paeschke, Katrin; Bochman, Matthew L.; Daniela Garcia, P.; Cejka, Petr; Friedman, Katherine L.; Kowalczykowski, Stephen C.; Zakian, Virginia A.
In: Nature, Vol. 497, No. 7450, 2013, p. 458-462.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pif1 family helicases suppress genome instability at G-quadruplex motifs
AU - Paeschke, Katrin
AU - Bochman, Matthew L.
AU - Daniela Garcia, P.
AU - Cejka, Petr
AU - Friedman, Katherine L.
AU - Kowalczykowski, Stephen C.
AU - Zakian, Virginia A.
PY - 2013
Y1 - 2013
N2 - The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.
AB - The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.
UR - http://www.scopus.com/inward/record.url?scp=84878122437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878122437&partnerID=8YFLogxK
U2 - 10.1038/nature12149
DO - 10.1038/nature12149
M3 - Article
C2 - 23657261
AN - SCOPUS:84878122437
VL - 497
SP - 458
EP - 462
JO - Nature
JF - Nature
SN - 0028-0836
IS - 7450
ER -