PI3K mediated electrotaxis of embryonic and adult neural progenitor cells in the presence of growth factors

Xiaoting Meng, Miguel Arocena, Josef Penninger, Fred H. Gage, Min Zhao, Bing Song

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Correct guidance of the migration of neural progenitor cells (NPCs) is essential for the development and repair of the central nervous system (CNS). Electric field (EF)-guided migration, electrotaxis, has been observed in many cell types. We report here that, in applied EFs of physiological magnitude, embryonic and adult NPCs show marked electrotaxis, which is dependent on the PI3K/Akt pathway. The electrotaxis was also evidenced by ex vivo investigation that transplanted NPCs migrated directionally towards cathode in organotypic spinal cord slice model when treated with EFs. Genetic disruption or pharmacological inhibition of phosphoinositide 3-kinase (PI3K) impaired electrotaxis, whereas EF exposure increased Akt phosphorylation in a growth factor-dependent manner and increased phosphatidylinositol-3,4,5-trisphosphate (PIP3) levels. EF treatments also induced asymmetric redistribution of PIP3, growth factor receptors, and actin cytoskeleton. Electrotaxis in both embryonic and adult NPCs requires epidermal growth factor (EGF) and fibroblast growth factor (FGF). Our results demonstrate the importance of the PI3K/Akt pathway in directed migration of NPCs driven by EFs and growth factors and highlight the potential of EFs to enhance the guidance of various NPC populations in CNS repair therapies.

Original languageEnglish (US)
Pages (from-to)210-217
Number of pages8
JournalExperimental Neurology
Volume227
Issue number1
DOIs
StatePublished - Jan 2011

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1-Phosphatidylinositol 4-Kinase
Intercellular Signaling Peptides and Proteins
Stem Cells
Central Nervous System
Growth Factor Receptors
Fibroblast Growth Factors
Actin Cytoskeleton
Epidermal Growth Factor
Spinal Cord
Electrodes
Phosphorylation
Pharmacology
Population

Keywords

  • Akt
  • Cell migration
  • Electric field
  • Growth factors
  • Neural progenitor cells
  • PI3K

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

PI3K mediated electrotaxis of embryonic and adult neural progenitor cells in the presence of growth factors. / Meng, Xiaoting; Arocena, Miguel; Penninger, Josef; Gage, Fred H.; Zhao, Min; Song, Bing.

In: Experimental Neurology, Vol. 227, No. 1, 01.2011, p. 210-217.

Research output: Contribution to journalArticle

Meng, Xiaoting ; Arocena, Miguel ; Penninger, Josef ; Gage, Fred H. ; Zhao, Min ; Song, Bing. / PI3K mediated electrotaxis of embryonic and adult neural progenitor cells in the presence of growth factors. In: Experimental Neurology. 2011 ; Vol. 227, No. 1. pp. 210-217.
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