Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence

Bomu Wu, Lena Hammar, Li Xing, Sevak Markarian, Jin Yan, Kenji Iwasaki, Yoshinori Fujiyoshi, Toshihiro Omura, R. Holland Cheng

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The structures of the double-shelled rice dwarf virus and of its single- shell core have been determined by cryoelectron microscopy and image reconstruction. The core carries a prominent density located at each of the icosahedral faces of its T = 1 lattice. These protrusions are formed by outer shell trimers, tightly inserted at the threefold positions of the core. Such configuration of the core may guide the assembly of the outer shell, aided by lateral interactions between its subunits, into a T = 13 lattice. The organization of the phytoreovirus capsid elucidates for the first time a general model for assembling two unique T numbers of quasi-equivalence.

Original languageEnglish (US)
Pages (from-to)18-25
Number of pages8
JournalVirology
Volume271
Issue number1
DOIs
StatePublished - May 25 2000
Externally publishedYes

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Reoviridae
Cryoelectron Microscopy
Computer-Assisted Image Processing
Capsid
Viruses
Oryza

Keywords

  • Computer image reconstruction
  • cryoEM
  • Quasi-equivalence
  • Reovirus
  • Rice dwarf virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Wu, B., Hammar, L., Xing, L., Markarian, S., Yan, J., Iwasaki, K., ... Cheng, R. H. (2000). Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence. Virology, 271(1), 18-25. https://doi.org/10.1006/viro.2000.0300

Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence. / Wu, Bomu; Hammar, Lena; Xing, Li; Markarian, Sevak; Yan, Jin; Iwasaki, Kenji; Fujiyoshi, Yoshinori; Omura, Toshihiro; Cheng, R. Holland.

In: Virology, Vol. 271, No. 1, 25.05.2000, p. 18-25.

Research output: Contribution to journalArticle

Wu, B, Hammar, L, Xing, L, Markarian, S, Yan, J, Iwasaki, K, Fujiyoshi, Y, Omura, T & Cheng, RH 2000, 'Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence', Virology, vol. 271, no. 1, pp. 18-25. https://doi.org/10.1006/viro.2000.0300
Wu, Bomu ; Hammar, Lena ; Xing, Li ; Markarian, Sevak ; Yan, Jin ; Iwasaki, Kenji ; Fujiyoshi, Yoshinori ; Omura, Toshihiro ; Cheng, R. Holland. / Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence. In: Virology. 2000 ; Vol. 271, No. 1. pp. 18-25.
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