Physiologic concentrations of arginine vasopressin activate human platelets in vitro

Theodore Wun, Teresa Paglieroni, Neil A. Lachant

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Arginine vasopressin (AVP) is a neurohypophyseal peptide hormone with protean effects. Previous reports had shown that AVP stimulates platelets, but only at concentrations 3-6 logs higher than the normal plasma concentrations in humans. In this study we tested the hypothesis that AVP, at physiologic concentrations, stimulated the expression of an activation-dependent platelet antigen. Platelets obtained from normal volunteers were incubated with increasing concentrations of AVP and the expression of the activation-dependent platelet antigen P-selectin (CD62) was determined by monoclonal antibodies and flow cytometry. There was a concentration-dependent increase in CD62 expression with increasing AVP concentration; at 1 pM AVP, 24.5% (1.3-88.5%) [median (range)] of platelets expressed CD62. The selective vasopressin V1 receptor antagonist d(CH2)5-Tyr(me)AVP (TM-AVP) completely abolished AVP-stimulated Cd62 expression. We conclude that AVP can activate platelets at concentrations found in normal humans, at least in vitro, and that this response is mediated by the platelet V1 receptor. AVP may be a physiologic platelet agonist.

Original languageEnglish (US)
Pages (from-to)968-972
Number of pages5
JournalBritish Journal of Haematology
Volume92
Issue number4
StatePublished - 1996

Fingerprint

Arginine Vasopressin
Blood Platelets
Posterior Pituitary Hormones
Vasopressin Receptors
Platelet Activation
In Vitro Techniques
Antigens
P-Selectin
Peptide Hormones
Healthy Volunteers
Flow Cytometry
Monoclonal Antibodies

Keywords

  • arginine vasopressin
  • flow cytometry
  • P-selectin

ASJC Scopus subject areas

  • Hematology

Cite this

Physiologic concentrations of arginine vasopressin activate human platelets in vitro. / Wun, Theodore; Paglieroni, Teresa; Lachant, Neil A.

In: British Journal of Haematology, Vol. 92, No. 4, 1996, p. 968-972.

Research output: Contribution to journalArticle

Wun, Theodore ; Paglieroni, Teresa ; Lachant, Neil A. / Physiologic concentrations of arginine vasopressin activate human platelets in vitro. In: British Journal of Haematology. 1996 ; Vol. 92, No. 4. pp. 968-972.
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