Physical properties of sponge aggregation factor. A unique proteoglycan complex

Charles B. Cauldwell, Pierre Henkart, Tom Humphreys

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


Physical properties of the aggregation factor isolated from the marine sponge Microciona parthena are described. The active molecular complex has an intrinsic viscosity of about 500 cm3/g and a sedimentation constant of 62 S. These results suggest a large, fibrous molecule of about 21 × 105 daltons. Light scattering yields a similar molecular weight and a radius of gyration of 800 Å. These data, together with the electron microscopic images presented in the accompanying paper, indicate that the aggregation factor complex in solution has an open, sunburst structure with a diameter of 1600 Å or more. When Ca2+ ions are removed, the factor complex gradually dissociates into glycoprotein subunit molecules of 2 × 105 daltons plus a core.

Original languageEnglish (US)
Pages (from-to)3051-3055
Number of pages5
Issue number16
StatePublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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