Abstract
Physical properties of the aggregation factor isolated from the marine sponge Microciona parthena are described. The active molecular complex has an intrinsic viscosity of about 500 cm3/g and a sedimentation constant of 62 S. These results suggest a large, fibrous molecule of about 21 × 105 daltons. Light scattering yields a similar molecular weight and a radius of gyration of 800 Å. These data, together with the electron microscopic images presented in the accompanying paper, indicate that the aggregation factor complex in solution has an open, sunburst structure with a diameter of 1600 Å or more. When Ca2+ ions are removed, the factor complex gradually dissociates into glycoprotein subunit molecules of 2 × 105 daltons plus a core.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3051-3055 |
| Number of pages | 5 |
| Journal | Biochemistry |
| Volume | 12 |
| Issue number | 16 |
| State | Published - 1973 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
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Physical properties of sponge aggregation factor. A unique proteoglycan complex. / Cauldwell, Charles B.; Henkart, Pierre; Humphreys, Tom.
In: Biochemistry, Vol. 12, No. 16, 1973, p. 3051-3055.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physical properties of sponge aggregation factor. A unique proteoglycan complex
AU - Cauldwell, Charles B.
AU - Henkart, Pierre
AU - Humphreys, Tom
PY - 1973
Y1 - 1973
N2 - Physical properties of the aggregation factor isolated from the marine sponge Microciona parthena are described. The active molecular complex has an intrinsic viscosity of about 500 cm3/g and a sedimentation constant of 62 S. These results suggest a large, fibrous molecule of about 21 × 105 daltons. Light scattering yields a similar molecular weight and a radius of gyration of 800 Å. These data, together with the electron microscopic images presented in the accompanying paper, indicate that the aggregation factor complex in solution has an open, sunburst structure with a diameter of 1600 Å or more. When Ca2+ ions are removed, the factor complex gradually dissociates into glycoprotein subunit molecules of 2 × 105 daltons plus a core.
AB - Physical properties of the aggregation factor isolated from the marine sponge Microciona parthena are described. The active molecular complex has an intrinsic viscosity of about 500 cm3/g and a sedimentation constant of 62 S. These results suggest a large, fibrous molecule of about 21 × 105 daltons. Light scattering yields a similar molecular weight and a radius of gyration of 800 Å. These data, together with the electron microscopic images presented in the accompanying paper, indicate that the aggregation factor complex in solution has an open, sunburst structure with a diameter of 1600 Å or more. When Ca2+ ions are removed, the factor complex gradually dissociates into glycoprotein subunit molecules of 2 × 105 daltons plus a core.
UR - http://www.scopus.com/inward/record.url?scp=0015882288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015882288&partnerID=8YFLogxK
M3 - Article
C2 - 4269810
AN - SCOPUS:0015882288
VL - 12
SP - 3051
EP - 3055
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 16
ER -