Physical, mechanical and degradation properties, and Schwann cell affinity of cross-linked chitosan films

Wenling Cao, Mingyu Cheng, Qiang Ao, Yandao Gong, Nanming Zhao, Xiufang Zhang

Research output: Contribution to journalArticlepeer-review

74 Scopus citations


Three kinds of cross-linked chitosan films were prepared with hexamethylene diisocyanate (HDI), epichlorohydrin (ECH) and glutaraldehyde (GA) as cross-linking agents, respectively. The physical and mechanical properties, biodegradability and Schwann cell affinity of the cross-linked films were investigated. A significant decrease in the degradation rate in lysozyme solution and a large change in the mechanical properties were observed compared with non-cross-linked chitosan films. The protein adsorption on chitosan films was determined by means of enzyme-linked immunosorbent assay (ELISA). In comparison with the non-cross-linked films, the chitosan films cross-linked with HDI showed a significant increase (up to 40-50%) in both fibronectin and laminin adsorption, while the protein adsorption on the other two kinds of cross-linked films was similar to that on non-cross-linked films. In addition, cell culture revealed that the HDI cross-linked chitosan films enhanced the spread and proliferation of Schwann cells while the other cross-linked films delayed the cell proliferation. These results suggest that HDI cross-linking of chitosan films provides a combination of physical properties, biodegradability and Schwann cell affinity suitable for peripheral nerve regeneration.

Original languageEnglish (US)
Pages (from-to)791-807
Number of pages17
JournalJournal of Biomaterials Science, Polymer Edition
Issue number6
StatePublished - Jun 1 2005
Externally publishedYes


  • Biodegradability
  • Chitosan
  • Cross-linking
  • Mechanical and physical properties
  • Peripheral nerve regeneration
  • Schwann cell

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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