Physical assignments of human chromosome 13 genes on pig chromosome 11 demonstrate extensive synteny and gene order conservation between pig and human

H. S. Sun, M. Yerle, P. Pinton, P. Chardon, C. Rogel-Gaillard, Leslie A Lyons, T. F. Laughlin, C. K. Tuggle

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previous mapping between the human and pig genomes suggested extensive conservation of human chromosome 13 (HSA13) to pig chromosome 11 (SSC11). The objectives of this study were comparative gene mapping of pig homologs of HSA13 genes and examining gene order within this conserved synteny group by physical assignment of each locus. A detailed HSA13 to SSC11 comparison was chosen since the comparative gene map is not well developed for these chromosomes and a rearranged gene order within conserved synteny groups was observed from the comparison between HSA13 and bovine chromosome 12 (BTA12). Heterologous primers for PCR were designed and used to amplify pig homologous fragments. The pig fragments were sequenced to confirm the homology. Six pig STSs (FLT1, ESD, RB1, HTR2A, EDNRB, and F10) were physically mapped using a somatic cell hybrid panel to SSC11, and fluorescent in situ hybridization (FISH) mapping was also applied to improve map resolution and determine gene order. Results from this study increase the comparative information available on SSC11 and suggest a conserved gene order on SSC11 and HSA13, in contrast to human:bovine comparisons of this syntenic group.

Original languageEnglish (US)
Pages (from-to)304-308
Number of pages5
JournalAnimal Genetics
Volume30
Issue number4
DOIs
StatePublished - Aug 1999
Externally publishedYes

Fingerprint

Synteny
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 11
Gene Order
Human Chromosomes
Swine
chromosomes
swine
Genes
genes
Chromosomes, Human, Pair 12
Hybrid Cells
Chromosome Mapping
cattle
Human Genome
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
somatic cells
chromosome mapping
Chromosomes

Keywords

  • Comparative map
  • Physical mapping
  • Pig
  • SSC11

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Physical assignments of human chromosome 13 genes on pig chromosome 11 demonstrate extensive synteny and gene order conservation between pig and human. / Sun, H. S.; Yerle, M.; Pinton, P.; Chardon, P.; Rogel-Gaillard, C.; Lyons, Leslie A; Laughlin, T. F.; Tuggle, C. K.

In: Animal Genetics, Vol. 30, No. 4, 08.1999, p. 304-308.

Research output: Contribution to journalArticle

Sun, H. S. ; Yerle, M. ; Pinton, P. ; Chardon, P. ; Rogel-Gaillard, C. ; Lyons, Leslie A ; Laughlin, T. F. ; Tuggle, C. K. / Physical assignments of human chromosome 13 genes on pig chromosome 11 demonstrate extensive synteny and gene order conservation between pig and human. In: Animal Genetics. 1999 ; Vol. 30, No. 4. pp. 304-308.
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