Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation

Yuval Blat, Reine U. Protacio, Neil Hunter, Nancy Kleckner

Research output: Contribution to journalArticle

228 Scopus citations

Abstract

Analysis of meiotic recombination by functional genomic approaches reveals prominent spatial and functional interactions among diverse organizational determinants. Recombination occurs between chromatin loop sequences; however, these sequences are spatially tethered to underlying chromosome axes via their recombinosomes. Meiotic chromosomal protein, Red1, localizes to chromosome axes; however, Red1 loading is modulated by R/G-bands isochores and thus by bulk chromatin state. Recombination is also modulated by isochore determinants: R-bands differentially favor double-strand break (DSB) formation but disfavor subsequent loading of meiotic RecA homolog, Dmc1. Red1 promotes DSB formation in both R- and G-bands and then promotes Dmc1 loading, specifically counteracting disfavoring R-band effects. These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels.

Original languageEnglish (US)
Pages (from-to)791-802
Number of pages12
JournalCell
Volume111
Issue number6
DOIs
StatePublished - Dec 13 2002

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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