Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation

Yuval Blat, Reine U. Protacio, Neil Hunter, Nancy Kleckner

Research output: Contribution to journalArticlepeer-review

248 Scopus citations

Abstract

Analysis of meiotic recombination by functional genomic approaches reveals prominent spatial and functional interactions among diverse organizational determinants. Recombination occurs between chromatin loop sequences; however, these sequences are spatially tethered to underlying chromosome axes via their recombinosomes. Meiotic chromosomal protein, Red1, localizes to chromosome axes; however, Red1 loading is modulated by R/G-bands isochores and thus by bulk chromatin state. Recombination is also modulated by isochore determinants: R-bands differentially favor double-strand break (DSB) formation but disfavor subsequent loading of meiotic RecA homolog, Dmc1. Red1 promotes DSB formation in both R- and G-bands and then promotes Dmc1 loading, specifically counteracting disfavoring R-band effects. These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels.

Original languageEnglish (US)
Pages (from-to)791-802
Number of pages12
JournalCell
Volume111
Issue number6
DOIs
StatePublished - Dec 13 2002

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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