Phylogenetic relationships of human and wildlife piroplasm isolates in the western United States inferred from the 18S nuclear small subunit RNA gene

A. M. Kjemtrup, J. Thomford, T. Robinson, Patricia A Conrad

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The 18S nuclear small subunit ribosomal RNA gene of piroplasms from wildlife and human cases of babesiosis in the western USA were isolated by PCR and sequenced. Phylogenetic analyses of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that piroplasm isolates from the human cases were indistinguishable from some of the isolates from the western wildlife species, most notably the isolates from mule deer (Odocoileus hemionus). These results suggest that large ungulates may serve as reservoirs for human piroplasm infection. The western piroplasm isolates from humans and wildlife formed a distinct clade, separate from other piroplasms found worldwide.

Original languageEnglish (US)
Pages (from-to)487-493
Number of pages7
JournalParasitology
Volume120
Issue number5
DOIs
StatePublished - 2000

Fingerprint

Small Nuclear RNA
Piroplasmida
Western United States
wildlife
RNA
phylogeny
Theileria
Odocoileus hemionus
Genes
Babesiosis
Babesia
Deer
genes
Equidae
rRNA Genes
Sequence Analysis
babesiosis
Polymerase Chain Reaction
ungulates
Infection

Keywords

  • Babesia
  • Phylogeny
  • Piroplasm
  • Theileria

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Phylogenetic relationships of human and wildlife piroplasm isolates in the western United States inferred from the 18S nuclear small subunit RNA gene. / Kjemtrup, A. M.; Thomford, J.; Robinson, T.; Conrad, Patricia A.

In: Parasitology, Vol. 120, No. 5, 2000, p. 487-493.

Research output: Contribution to journalArticle

@article{cc4edb29a57144b094d6362b1f7d2d78,
title = "Phylogenetic relationships of human and wildlife piroplasm isolates in the western United States inferred from the 18S nuclear small subunit RNA gene",
abstract = "The 18S nuclear small subunit ribosomal RNA gene of piroplasms from wildlife and human cases of babesiosis in the western USA were isolated by PCR and sequenced. Phylogenetic analyses of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that piroplasm isolates from the human cases were indistinguishable from some of the isolates from the western wildlife species, most notably the isolates from mule deer (Odocoileus hemionus). These results suggest that large ungulates may serve as reservoirs for human piroplasm infection. The western piroplasm isolates from humans and wildlife formed a distinct clade, separate from other piroplasms found worldwide.",
keywords = "Babesia, Phylogeny, Piroplasm, Theileria",
author = "Kjemtrup, {A. M.} and J. Thomford and T. Robinson and Conrad, {Patricia A}",
year = "2000",
doi = "10.1017/S003118209900582X",
language = "English (US)",
volume = "120",
pages = "487--493",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "5",

}

TY - JOUR

T1 - Phylogenetic relationships of human and wildlife piroplasm isolates in the western United States inferred from the 18S nuclear small subunit RNA gene

AU - Kjemtrup, A. M.

AU - Thomford, J.

AU - Robinson, T.

AU - Conrad, Patricia A

PY - 2000

Y1 - 2000

N2 - The 18S nuclear small subunit ribosomal RNA gene of piroplasms from wildlife and human cases of babesiosis in the western USA were isolated by PCR and sequenced. Phylogenetic analyses of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that piroplasm isolates from the human cases were indistinguishable from some of the isolates from the western wildlife species, most notably the isolates from mule deer (Odocoileus hemionus). These results suggest that large ungulates may serve as reservoirs for human piroplasm infection. The western piroplasm isolates from humans and wildlife formed a distinct clade, separate from other piroplasms found worldwide.

AB - The 18S nuclear small subunit ribosomal RNA gene of piroplasms from wildlife and human cases of babesiosis in the western USA were isolated by PCR and sequenced. Phylogenetic analyses of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that piroplasm isolates from the human cases were indistinguishable from some of the isolates from the western wildlife species, most notably the isolates from mule deer (Odocoileus hemionus). These results suggest that large ungulates may serve as reservoirs for human piroplasm infection. The western piroplasm isolates from humans and wildlife formed a distinct clade, separate from other piroplasms found worldwide.

KW - Babesia

KW - Phylogeny

KW - Piroplasm

KW - Theileria

UR - http://www.scopus.com/inward/record.url?scp=0034095492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034095492&partnerID=8YFLogxK

U2 - 10.1017/S003118209900582X

DO - 10.1017/S003118209900582X

M3 - Article

VL - 120

SP - 487

EP - 493

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 5

ER -