Phylogenetic analysis of marine mammal herpesviruses

Heather T D Maness, Hendrik H. Nollens, Eric D. Jensen, Tracey Goldstein, Sarah LaMere, April Childress, John Sykes, Judy St. Leger, Géraldine Lacave, F. Ed Latson, James F X Wellehan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Five novel DNA-dependent DNA polymerase (Dpol) herpesviral sequences were generated using nested consensus polymerase chain reaction (PCR) in clinical samples from a harbor seal (Phoca vitulina), bottlenose dolphin (Tursiops truncatus), orca (Orcinus orca), California sea lion (Zalophus californianus), and a Phocid herpesvirus 2 (PhHV-2) isolate from a harbor seal (used as positive control). These novel sequences and other representative herpesvirus sequences were included in Bayesian and Maximum Likelihood analyses to illustrate the phylogeny of herpesviruses amongst the marine mammal host species and in comparison to those of other animals. All 19 novel and known marine mammal herpesviruses included in the analyses aligned with members of the Alphaherpesvirinae or Gammaherpesvirinae subfamilies. The novel harbor seal herpesvirus clustered with members of the Macavirus genus, subfamily Gammaherpesvirinae. The novel bottlenose dolphin herpesvirus clustered together in a monophyletic group with another delphinid alphaherpesvirus but could not be associated with an established genus. The orca herpesvirus also clustered with a delphinid alphaherpesvirus and formed a separate clade. The sea lion herpesvirus clustered with PhHV-2. PhHV-1 clustered with varicelloviruses and PhHV-2 clustered strongly in the Gammaherpesvirinae genus Percavirus. All cetacean gammaherpesviruses formed a monophyletic clade and could not be associated with an established gammaherpesviral genus.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalVeterinary Microbiology
Volume149
Issue number1-2
DOIs
StatePublished - Apr 21 2011

Fingerprint

Phocid herpesvirus 2
Gammaherpesvirinae
Phoca vitulina
Tursiops truncatus
Herpesviridae
marine mammals
Mammals
Percavirus
Macavirus
Phocid herpesvirus 1
Varicellovirus
Phoca
phylogeny
Alphaherpesvirinae
Sea Lions
Bottle-Nosed Dolphin
Orcinus orca
Otariidae
DNA-directed DNA polymerase
polymerase chain reaction

Keywords

  • Cetacean
  • Delphinid
  • Herpesviridae
  • Herpesvirus
  • Marine mammal
  • Otariid
  • Phocid
  • Phylogeny
  • Pinniped
  • Taxonomy

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Maness, H. T. D., Nollens, H. H., Jensen, E. D., Goldstein, T., LaMere, S., Childress, A., ... Wellehan, J. F. X. (2011). Phylogenetic analysis of marine mammal herpesviruses. Veterinary Microbiology, 149(1-2), 23-29. https://doi.org/10.1016/j.vetmic.2010.09.035

Phylogenetic analysis of marine mammal herpesviruses. / Maness, Heather T D; Nollens, Hendrik H.; Jensen, Eric D.; Goldstein, Tracey; LaMere, Sarah; Childress, April; Sykes, John; St. Leger, Judy; Lacave, Géraldine; Ed Latson, F.; Wellehan, James F X.

In: Veterinary Microbiology, Vol. 149, No. 1-2, 21.04.2011, p. 23-29.

Research output: Contribution to journalArticle

Maness, HTD, Nollens, HH, Jensen, ED, Goldstein, T, LaMere, S, Childress, A, Sykes, J, St. Leger, J, Lacave, G, Ed Latson, F & Wellehan, JFX 2011, 'Phylogenetic analysis of marine mammal herpesviruses', Veterinary Microbiology, vol. 149, no. 1-2, pp. 23-29. https://doi.org/10.1016/j.vetmic.2010.09.035
Maness HTD, Nollens HH, Jensen ED, Goldstein T, LaMere S, Childress A et al. Phylogenetic analysis of marine mammal herpesviruses. Veterinary Microbiology. 2011 Apr 21;149(1-2):23-29. https://doi.org/10.1016/j.vetmic.2010.09.035
Maness, Heather T D ; Nollens, Hendrik H. ; Jensen, Eric D. ; Goldstein, Tracey ; LaMere, Sarah ; Childress, April ; Sykes, John ; St. Leger, Judy ; Lacave, Géraldine ; Ed Latson, F. ; Wellehan, James F X. / Phylogenetic analysis of marine mammal herpesviruses. In: Veterinary Microbiology. 2011 ; Vol. 149, No. 1-2. pp. 23-29.
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