Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene

Nien Tsu Chen, Yasuko Kuwabara, Christopher Conley, Yi Chun Liao, Shiao Ya Hong, Michelle Chen, Yi Ping Shih, Hongwu Chen, Fushing Hsieh, Su Hao Lo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cten is a focal adhesion molecule and a member of the tensin family. Its expression is highly enriched in the prostate and placenta, suggesting that cten gene might be closely associated with mammalian species. Recent studies have reported that cten expression is frequently up-regulated in a variety of cancers and its levels appear to correlate with tumorigenicity. Here, we have (1) analyzed cten sequences of various species to build a phylogenetic tree, (2) examined cten mRNA levels in human and mouse tissues to establish its expression profiles, and (3) determined the promoter region of human CTEN gene in cell lines and in a mouse model to understand its transcriptional regulation. Our analyses indicate that all currently known cten genes are present in mammals. The prostate and placenta are the two most cten abundant tissues in human and mouse, meanwhile brain and lung also express low levels of cten. Results from cell culture reporter assays demonstrate that a 327-bp fragment is the shortest functional promoter. All functional promoter constructs produce 40- to 160-fold increases in luciferase reporter activities in normal prostate cells, whereas lower activities (<40-fold) are detected in non-prostatic cell lines. To evaluate CTEN promoter activity in mice and develop a new tissue specific Cre recombinase mouse model, we have established pCTEN-Cre:R26R mice by crossing R26R β-galactosidase reporter mice with pCTEN-Cre transgenic mice, in which the 327-bp cten promoter drives the expression of Cre recombinase. X-gal analysis has shown strong β-galactosidase activities in the prostate, brain, and few other tissues in pCTEN-Cre:R26R mice. Altogether, we have identified the promoter region of human cten gene and provided a useful tool for investigating cell lineages and generating tissue-specific knockout or knockin mice.

Original languageEnglish (US)
Pages (from-to)90-97
Number of pages8
JournalGene
Volume520
Issue number2
DOIs
StatePublished - May 15 2013

Fingerprint

Genes
Prostate
Galactosidases
Genetic Promoter Regions
Placenta
Cell Line
Focal Adhesions
Brain
Cell Lineage
Luciferases
Transgenic Mice
Mammals
Cell Culture Techniques
Lung
Messenger RNA
Neoplasms
Cre recombinase

Keywords

  • Cre mouse
  • Cten/Tns4
  • Phylogenetic
  • Promoter

ASJC Scopus subject areas

  • Genetics

Cite this

Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene. / Chen, Nien Tsu; Kuwabara, Yasuko; Conley, Christopher; Liao, Yi Chun; Hong, Shiao Ya; Chen, Michelle; Shih, Yi Ping; Chen, Hongwu; Hsieh, Fushing; Lo, Su Hao.

In: Gene, Vol. 520, No. 2, 15.05.2013, p. 90-97.

Research output: Contribution to journalArticle

Chen, NT, Kuwabara, Y, Conley, C, Liao, YC, Hong, SY, Chen, M, Shih, YP, Chen, H, Hsieh, F & Lo, SH 2013, 'Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene', Gene, vol. 520, no. 2, pp. 90-97. https://doi.org/10.1016/j.gene.2013.02.041
Chen NT, Kuwabara Y, Conley C, Liao YC, Hong SY, Chen M et al. Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene. Gene. 2013 May 15;520(2):90-97. https://doi.org/10.1016/j.gene.2013.02.041
Chen, Nien Tsu ; Kuwabara, Yasuko ; Conley, Christopher ; Liao, Yi Chun ; Hong, Shiao Ya ; Chen, Michelle ; Shih, Yi Ping ; Chen, Hongwu ; Hsieh, Fushing ; Lo, Su Hao. / Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene. In: Gene. 2013 ; Vol. 520, No. 2. pp. 90-97.
@article{b0eaff3c69b3483ea724cdd2a344f002,
title = "Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene",
abstract = "Cten is a focal adhesion molecule and a member of the tensin family. Its expression is highly enriched in the prostate and placenta, suggesting that cten gene might be closely associated with mammalian species. Recent studies have reported that cten expression is frequently up-regulated in a variety of cancers and its levels appear to correlate with tumorigenicity. Here, we have (1) analyzed cten sequences of various species to build a phylogenetic tree, (2) examined cten mRNA levels in human and mouse tissues to establish its expression profiles, and (3) determined the promoter region of human CTEN gene in cell lines and in a mouse model to understand its transcriptional regulation. Our analyses indicate that all currently known cten genes are present in mammals. The prostate and placenta are the two most cten abundant tissues in human and mouse, meanwhile brain and lung also express low levels of cten. Results from cell culture reporter assays demonstrate that a 327-bp fragment is the shortest functional promoter. All functional promoter constructs produce 40- to 160-fold increases in luciferase reporter activities in normal prostate cells, whereas lower activities (<40-fold) are detected in non-prostatic cell lines. To evaluate CTEN promoter activity in mice and develop a new tissue specific Cre recombinase mouse model, we have established pCTEN-Cre:R26R mice by crossing R26R β-galactosidase reporter mice with pCTEN-Cre transgenic mice, in which the 327-bp cten promoter drives the expression of Cre recombinase. X-gal analysis has shown strong β-galactosidase activities in the prostate, brain, and few other tissues in pCTEN-Cre:R26R mice. Altogether, we have identified the promoter region of human cten gene and provided a useful tool for investigating cell lineages and generating tissue-specific knockout or knockin mice.",
keywords = "Cre mouse, Cten/Tns4, Phylogenetic, Promoter",
author = "Chen, {Nien Tsu} and Yasuko Kuwabara and Christopher Conley and Liao, {Yi Chun} and Hong, {Shiao Ya} and Michelle Chen and Shih, {Yi Ping} and Hongwu Chen and Fushing Hsieh and Lo, {Su Hao}",
year = "2013",
month = "5",
day = "15",
doi = "10.1016/j.gene.2013.02.041",
language = "English (US)",
volume = "520",
pages = "90--97",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Phylogenetic analysis, expression patterns, and transcriptional regulation of human CTEN gene

AU - Chen, Nien Tsu

AU - Kuwabara, Yasuko

AU - Conley, Christopher

AU - Liao, Yi Chun

AU - Hong, Shiao Ya

AU - Chen, Michelle

AU - Shih, Yi Ping

AU - Chen, Hongwu

AU - Hsieh, Fushing

AU - Lo, Su Hao

PY - 2013/5/15

Y1 - 2013/5/15

N2 - Cten is a focal adhesion molecule and a member of the tensin family. Its expression is highly enriched in the prostate and placenta, suggesting that cten gene might be closely associated with mammalian species. Recent studies have reported that cten expression is frequently up-regulated in a variety of cancers and its levels appear to correlate with tumorigenicity. Here, we have (1) analyzed cten sequences of various species to build a phylogenetic tree, (2) examined cten mRNA levels in human and mouse tissues to establish its expression profiles, and (3) determined the promoter region of human CTEN gene in cell lines and in a mouse model to understand its transcriptional regulation. Our analyses indicate that all currently known cten genes are present in mammals. The prostate and placenta are the two most cten abundant tissues in human and mouse, meanwhile brain and lung also express low levels of cten. Results from cell culture reporter assays demonstrate that a 327-bp fragment is the shortest functional promoter. All functional promoter constructs produce 40- to 160-fold increases in luciferase reporter activities in normal prostate cells, whereas lower activities (<40-fold) are detected in non-prostatic cell lines. To evaluate CTEN promoter activity in mice and develop a new tissue specific Cre recombinase mouse model, we have established pCTEN-Cre:R26R mice by crossing R26R β-galactosidase reporter mice with pCTEN-Cre transgenic mice, in which the 327-bp cten promoter drives the expression of Cre recombinase. X-gal analysis has shown strong β-galactosidase activities in the prostate, brain, and few other tissues in pCTEN-Cre:R26R mice. Altogether, we have identified the promoter region of human cten gene and provided a useful tool for investigating cell lineages and generating tissue-specific knockout or knockin mice.

AB - Cten is a focal adhesion molecule and a member of the tensin family. Its expression is highly enriched in the prostate and placenta, suggesting that cten gene might be closely associated with mammalian species. Recent studies have reported that cten expression is frequently up-regulated in a variety of cancers and its levels appear to correlate with tumorigenicity. Here, we have (1) analyzed cten sequences of various species to build a phylogenetic tree, (2) examined cten mRNA levels in human and mouse tissues to establish its expression profiles, and (3) determined the promoter region of human CTEN gene in cell lines and in a mouse model to understand its transcriptional regulation. Our analyses indicate that all currently known cten genes are present in mammals. The prostate and placenta are the two most cten abundant tissues in human and mouse, meanwhile brain and lung also express low levels of cten. Results from cell culture reporter assays demonstrate that a 327-bp fragment is the shortest functional promoter. All functional promoter constructs produce 40- to 160-fold increases in luciferase reporter activities in normal prostate cells, whereas lower activities (<40-fold) are detected in non-prostatic cell lines. To evaluate CTEN promoter activity in mice and develop a new tissue specific Cre recombinase mouse model, we have established pCTEN-Cre:R26R mice by crossing R26R β-galactosidase reporter mice with pCTEN-Cre transgenic mice, in which the 327-bp cten promoter drives the expression of Cre recombinase. X-gal analysis has shown strong β-galactosidase activities in the prostate, brain, and few other tissues in pCTEN-Cre:R26R mice. Altogether, we have identified the promoter region of human cten gene and provided a useful tool for investigating cell lineages and generating tissue-specific knockout or knockin mice.

KW - Cre mouse

KW - Cten/Tns4

KW - Phylogenetic

KW - Promoter

UR - http://www.scopus.com/inward/record.url?scp=84876305968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876305968&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2013.02.041

DO - 10.1016/j.gene.2013.02.041

M3 - Article

C2 - 23500447

AN - SCOPUS:84876305968

VL - 520

SP - 90

EP - 97

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -