Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit

P. D. Calvert, N. V. Krasnoperova, A. L. Lyubarsky, T. Isayama, M. Nicoló, B. Kosaras, G. Wong, K. S. Gannon, R. F. Margolskee, R. L. Sidman, Edward N Pugh Jr, C. L. Makino, J. Lem

Research output: Contribution to journalArticle

249 Citations (Scopus)

Abstract

Retinal photoreceptors use the heterotrimeric G protein transducin to couple rhodopsin to a biochemical cascade that underlies the electrical photoresponse. Several isoforms of each transducin subunit are present in the retina. Although rods and cones seem to contain distinct transducin subunits, it is not known whether phototransduction in a given cell type depends strictly on a single form of each subunit. To approach this question, we have deleted the gene for the rod transducin α-subunit in mice. In hemizygous knockout mice, there was a small reduction in retinal transducin α-subunit content but retinal morphology and the physiology of single rods were largely normal. In homozygous knockout mice, a mild retinal degeneration occurred with age. Rod-driven components were absent from the electroretinogram, whereas cone-driven components were retained. Every photoreceptor examined by single-cell recording failed to respond to flashes, with one exception. The solitary responsive cell was insensitive, as expected for a cone, but had a rod-like spectral sensitivity and flash response kinetics that were slow, even for rods. These results indicate that most if not all rods use a single transducin type in phototransduction.

Original languageEnglish (US)
Pages (from-to)13913-13918
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number25
DOIs
StatePublished - Dec 5 2000
Externally publishedYes

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Transducin
Light Signal Transduction
Transgenic Mice
Vertebrate Photoreceptor Cells
Knockout Mice
Heterotrimeric GTP-Binding Proteins
Retinal Degeneration
Rhodopsin
Retina
Protein Isoforms
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit. / Calvert, P. D.; Krasnoperova, N. V.; Lyubarsky, A. L.; Isayama, T.; Nicoló, M.; Kosaras, B.; Wong, G.; Gannon, K. S.; Margolskee, R. F.; Sidman, R. L.; Pugh Jr, Edward N; Makino, C. L.; Lem, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 25, 05.12.2000, p. 13913-13918.

Research output: Contribution to journalArticle

Calvert, PD, Krasnoperova, NV, Lyubarsky, AL, Isayama, T, Nicoló, M, Kosaras, B, Wong, G, Gannon, KS, Margolskee, RF, Sidman, RL, Pugh Jr, EN, Makino, CL & Lem, J 2000, 'Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit', Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 25, pp. 13913-13918. https://doi.org/10.1073/pnas.250478897
Calvert, P. D. ; Krasnoperova, N. V. ; Lyubarsky, A. L. ; Isayama, T. ; Nicoló, M. ; Kosaras, B. ; Wong, G. ; Gannon, K. S. ; Margolskee, R. F. ; Sidman, R. L. ; Pugh Jr, Edward N ; Makino, C. L. ; Lem, J. / Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 25. pp. 13913-13918.
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