Photoreceptor layer thickness changes during dark adaptation observed with ultrahigh-resolution optical coherence tomography

Chen D. Lu, Byungkun Lee, Julia Schottenhamml, Andreas Maier, Edward N Pugh Jr, James G. Fujimoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

PURPOSE. To examine outer retinal band changes after flash stimulus and subsequent dark adaptation with ultrahigh-resolution optical coherence tomography (UHR-OCT). METHODS. Five dark-adapted left eyes of five normal subjects were imaged with 3-μm axialresolution UHR-OCT during 30 minutes of dark adaptation following 96%, 54%, 23%, and 0% full-field and 54% half-field rhodopsin bleach. We identified the ellipsoid zone inner segment/outer segment (EZ[IS/OS]), cone interdigitation zone (CIZ), rod interdigitation zone (RIZ), retinal pigment epithelium (RPE), and Bruch’s membrane (BM) axial positions and generated two-dimensional thickness maps of the EZ(IS/OS) to the four bands. The average thickness over an area of the thickness map was compared against that of the dark-adapted baselines. The time-dependent thickness changes (photoresponses) were statistically compared against 0% bleach. Dark adaptometry was performed with the same bleaching protocol. RESULTS. The EZ(IS/OS)-CIZ photoresponse was significantly different at 96% (P < 0.0001) and 54% (P = 0.006) bleach. At all three bleaching levels, the EZ(IS/OS)-RIZ, -RPE, and -BM responses were significantly different (P < 0.0001). The EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ time courses were similar to the recovery of rod- and cone-mediated sensitivity, respectively, measured with dark adaptometry. The maximal EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ response magnitudes doubled from 54% to 96% bleach. Both EZ(IS/OS)-RPE and EZ(IS/OS)-BM responses resembled dampened oscillations that were graded in amplitude and duration with bleaching intensity. Half-field photoresponses were localized to the stimulated retina. CONCLUSIONS. With noninvasive, near-infrared UHR-OCT, we characterized three distinct, spatially localized photoresponses in the outer retinal bands. These photoresponses have potential value as physical correlates of photoreceptor function.

Original languageEnglish (US)
Pages (from-to)4632-4643
Number of pages12
JournalInvestigative Ophthalmology and Visual Science
Volume58
Issue number11
DOIs
StatePublished - Sep 1 2017

Fingerprint

Bruch Membrane
Dark Adaptation
Rod Cell Outer Segment
Retinal Pigment Epithelium
Optical Coherence Tomography
Vertebrate Photoreceptor Cells
Rhodopsin
Retina

Keywords

  • Dark adaptation
  • Optical coherence tomography
  • Outer segments
  • Photoreceptors

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Photoreceptor layer thickness changes during dark adaptation observed with ultrahigh-resolution optical coherence tomography. / Lu, Chen D.; Lee, Byungkun; Schottenhamml, Julia; Maier, Andreas; Pugh Jr, Edward N; Fujimoto, James G.

In: Investigative Ophthalmology and Visual Science, Vol. 58, No. 11, 01.09.2017, p. 4632-4643.

Research output: Contribution to journalArticle

Lu, Chen D. ; Lee, Byungkun ; Schottenhamml, Julia ; Maier, Andreas ; Pugh Jr, Edward N ; Fujimoto, James G. / Photoreceptor layer thickness changes during dark adaptation observed with ultrahigh-resolution optical coherence tomography. In: Investigative Ophthalmology and Visual Science. 2017 ; Vol. 58, No. 11. pp. 4632-4643.
@article{d33e33015d86485cae95d8055401f892,
title = "Photoreceptor layer thickness changes during dark adaptation observed with ultrahigh-resolution optical coherence tomography",
abstract = "PURPOSE. To examine outer retinal band changes after flash stimulus and subsequent dark adaptation with ultrahigh-resolution optical coherence tomography (UHR-OCT). METHODS. Five dark-adapted left eyes of five normal subjects were imaged with 3-μm axialresolution UHR-OCT during 30 minutes of dark adaptation following 96{\%}, 54{\%}, 23{\%}, and 0{\%} full-field and 54{\%} half-field rhodopsin bleach. We identified the ellipsoid zone inner segment/outer segment (EZ[IS/OS]), cone interdigitation zone (CIZ), rod interdigitation zone (RIZ), retinal pigment epithelium (RPE), and Bruch’s membrane (BM) axial positions and generated two-dimensional thickness maps of the EZ(IS/OS) to the four bands. The average thickness over an area of the thickness map was compared against that of the dark-adapted baselines. The time-dependent thickness changes (photoresponses) were statistically compared against 0{\%} bleach. Dark adaptometry was performed with the same bleaching protocol. RESULTS. The EZ(IS/OS)-CIZ photoresponse was significantly different at 96{\%} (P < 0.0001) and 54{\%} (P = 0.006) bleach. At all three bleaching levels, the EZ(IS/OS)-RIZ, -RPE, and -BM responses were significantly different (P < 0.0001). The EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ time courses were similar to the recovery of rod- and cone-mediated sensitivity, respectively, measured with dark adaptometry. The maximal EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ response magnitudes doubled from 54{\%} to 96{\%} bleach. Both EZ(IS/OS)-RPE and EZ(IS/OS)-BM responses resembled dampened oscillations that were graded in amplitude and duration with bleaching intensity. Half-field photoresponses were localized to the stimulated retina. CONCLUSIONS. With noninvasive, near-infrared UHR-OCT, we characterized three distinct, spatially localized photoresponses in the outer retinal bands. These photoresponses have potential value as physical correlates of photoreceptor function.",
keywords = "Dark adaptation, Optical coherence tomography, Outer segments, Photoreceptors",
author = "Lu, {Chen D.} and Byungkun Lee and Julia Schottenhamml and Andreas Maier and {Pugh Jr}, {Edward N} and Fujimoto, {James G.}",
year = "2017",
month = "9",
day = "1",
doi = "10.1167/iovs.17-22171",
language = "English (US)",
volume = "58",
pages = "4632--4643",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "11",

}

TY - JOUR

T1 - Photoreceptor layer thickness changes during dark adaptation observed with ultrahigh-resolution optical coherence tomography

AU - Lu, Chen D.

AU - Lee, Byungkun

AU - Schottenhamml, Julia

AU - Maier, Andreas

AU - Pugh Jr, Edward N

AU - Fujimoto, James G.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - PURPOSE. To examine outer retinal band changes after flash stimulus and subsequent dark adaptation with ultrahigh-resolution optical coherence tomography (UHR-OCT). METHODS. Five dark-adapted left eyes of five normal subjects were imaged with 3-μm axialresolution UHR-OCT during 30 minutes of dark adaptation following 96%, 54%, 23%, and 0% full-field and 54% half-field rhodopsin bleach. We identified the ellipsoid zone inner segment/outer segment (EZ[IS/OS]), cone interdigitation zone (CIZ), rod interdigitation zone (RIZ), retinal pigment epithelium (RPE), and Bruch’s membrane (BM) axial positions and generated two-dimensional thickness maps of the EZ(IS/OS) to the four bands. The average thickness over an area of the thickness map was compared against that of the dark-adapted baselines. The time-dependent thickness changes (photoresponses) were statistically compared against 0% bleach. Dark adaptometry was performed with the same bleaching protocol. RESULTS. The EZ(IS/OS)-CIZ photoresponse was significantly different at 96% (P < 0.0001) and 54% (P = 0.006) bleach. At all three bleaching levels, the EZ(IS/OS)-RIZ, -RPE, and -BM responses were significantly different (P < 0.0001). The EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ time courses were similar to the recovery of rod- and cone-mediated sensitivity, respectively, measured with dark adaptometry. The maximal EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ response magnitudes doubled from 54% to 96% bleach. Both EZ(IS/OS)-RPE and EZ(IS/OS)-BM responses resembled dampened oscillations that were graded in amplitude and duration with bleaching intensity. Half-field photoresponses were localized to the stimulated retina. CONCLUSIONS. With noninvasive, near-infrared UHR-OCT, we characterized three distinct, spatially localized photoresponses in the outer retinal bands. These photoresponses have potential value as physical correlates of photoreceptor function.

AB - PURPOSE. To examine outer retinal band changes after flash stimulus and subsequent dark adaptation with ultrahigh-resolution optical coherence tomography (UHR-OCT). METHODS. Five dark-adapted left eyes of five normal subjects were imaged with 3-μm axialresolution UHR-OCT during 30 minutes of dark adaptation following 96%, 54%, 23%, and 0% full-field and 54% half-field rhodopsin bleach. We identified the ellipsoid zone inner segment/outer segment (EZ[IS/OS]), cone interdigitation zone (CIZ), rod interdigitation zone (RIZ), retinal pigment epithelium (RPE), and Bruch’s membrane (BM) axial positions and generated two-dimensional thickness maps of the EZ(IS/OS) to the four bands. The average thickness over an area of the thickness map was compared against that of the dark-adapted baselines. The time-dependent thickness changes (photoresponses) were statistically compared against 0% bleach. Dark adaptometry was performed with the same bleaching protocol. RESULTS. The EZ(IS/OS)-CIZ photoresponse was significantly different at 96% (P < 0.0001) and 54% (P = 0.006) bleach. At all three bleaching levels, the EZ(IS/OS)-RIZ, -RPE, and -BM responses were significantly different (P < 0.0001). The EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ time courses were similar to the recovery of rod- and cone-mediated sensitivity, respectively, measured with dark adaptometry. The maximal EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ response magnitudes doubled from 54% to 96% bleach. Both EZ(IS/OS)-RPE and EZ(IS/OS)-BM responses resembled dampened oscillations that were graded in amplitude and duration with bleaching intensity. Half-field photoresponses were localized to the stimulated retina. CONCLUSIONS. With noninvasive, near-infrared UHR-OCT, we characterized three distinct, spatially localized photoresponses in the outer retinal bands. These photoresponses have potential value as physical correlates of photoreceptor function.

KW - Dark adaptation

KW - Optical coherence tomography

KW - Outer segments

KW - Photoreceptors

UR - http://www.scopus.com/inward/record.url?scp=85029414802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029414802&partnerID=8YFLogxK

U2 - 10.1167/iovs.17-22171

DO - 10.1167/iovs.17-22171

M3 - Article

C2 - 28898357

AN - SCOPUS:85029414802

VL - 58

SP - 4632

EP - 4643

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 11

ER -