Photoreceptor disc shedding in the living human eye

Omer P. Kocaoglu, Zhuolin Liu, Furu Zhang, Kazuhiro Kurokawa, Ravi Jonnal, Donald T. Miller

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cone photoreceptors undergo a daily cycle of renewal and shedding of membranous discs in their outer segments (OS), the portion responsible for light capture. These physiological processes are fundamental to maintaining photoreceptor health, and their dysfunction is associated with numerous retinal disease. While both processes have been extensively studied in animal models and postmortem eyes, little is known about them in the living eye, in particular human. In this study, we report discovery of the optical signature associated with disc shedding using a method based on adaptive optics optical coherence tomography (AO-OCT) in conjunction with post-processing methods to track and monitor individual cone cells in 4D. The optical signature of disc shedding is characterized by an abrupt transient loss in the cone outer segment tip (COST) reflection followed by its return that is axially displaced anteriorly. Using this signature, we measured the temporal and spatial properties of shedding events in three normal subjects. Average duration of the shedding event was 8.8 ± 13.4 minutes, and average length loss of the OS was 2.1 μm (7.0% of OS length). Prevalence of cone shedding was highest in the morning (14.3%) followed by the afternoon (5.7%) and evening (4.0%), with load distributed across the imaged patch. To the best of our knowledge these are the first images of photoreceptor disc shedding in the living retina.

Original languageEnglish (US)
Article number#274076
Pages (from-to)4554-4568
Number of pages15
JournalBiomedical Optics Express
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2016

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photoreceptors
Physiological Phenomena
Retinal Cone Photoreceptor Cells
Retinal Diseases
Optical Coherence Tomography
cones
Retina
Animal Models
Light
signatures
Health
evening
animal models
morning
retina
adaptive optics
health
monitors
tomography
cycles

Keywords

  • Active or adaptive optics
  • Ophthalmology
  • Optical coherence tomography
  • Vision - photoreceptors
  • Vision system - noninvasive assessment
  • Visual optics, receptor optics

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Kocaoglu, O. P., Liu, Z., Zhang, F., Kurokawa, K., Jonnal, R., & Miller, D. T. (2016). Photoreceptor disc shedding in the living human eye. Biomedical Optics Express, 7(11), 4554-4568. [#274076]. https://doi.org/10.1364/BOE.7.004554

Photoreceptor disc shedding in the living human eye. / Kocaoglu, Omer P.; Liu, Zhuolin; Zhang, Furu; Kurokawa, Kazuhiro; Jonnal, Ravi; Miller, Donald T.

In: Biomedical Optics Express, Vol. 7, No. 11, #274076, 01.11.2016, p. 4554-4568.

Research output: Contribution to journalArticle

Kocaoglu, OP, Liu, Z, Zhang, F, Kurokawa, K, Jonnal, R & Miller, DT 2016, 'Photoreceptor disc shedding in the living human eye', Biomedical Optics Express, vol. 7, no. 11, #274076, pp. 4554-4568. https://doi.org/10.1364/BOE.7.004554
Kocaoglu OP, Liu Z, Zhang F, Kurokawa K, Jonnal R, Miller DT. Photoreceptor disc shedding in the living human eye. Biomedical Optics Express. 2016 Nov 1;7(11):4554-4568. #274076. https://doi.org/10.1364/BOE.7.004554
Kocaoglu, Omer P. ; Liu, Zhuolin ; Zhang, Furu ; Kurokawa, Kazuhiro ; Jonnal, Ravi ; Miller, Donald T. / Photoreceptor disc shedding in the living human eye. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 11. pp. 4554-4568.
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