Photopatternable and photoactive hydrogel for on-demand generation of hydrogen peroxide in cell culture

Shaun P. Garland, Royal Y. Wang, Vijay Krishna Raghunathan, Kit Lam, Christopher J Murphy, Paul Russell, Gang Sun, Tingrui Pan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Oxidative stress, largely mediated by reactive oxygen species (ROS), is a nearly ubiquitous component in complex biological processes such as aging and disease. Optimal invitro methods used in elucidating disease mechanisms would deliver of low levels of hydrogen peroxide, emulating the invivo pathological state, but current methods are limited by kinetic stability or accurate measurement of the dose administered. Here we present an invitro platform that exploits anthraquinone catalysts for the photocatalytic production of hydrogen peroxide. This system can be dynamically tuned to provide constant generation of hydrogen peroxide at a desired physiologic rate over at least 14 days and is described using a kinetic model. Material characterization and stability is discussed along with a proof-of-concept invitro study that assessed the viability of cells as they were oxidatively challenged over 24h at different ROS generation rates.

Original languageEnglish (US)
Pages (from-to)1762-1770
Number of pages9
JournalBiomaterials
Volume35
Issue number5
DOIs
StatePublished - Feb 2014

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Keywords

  • Anthraquinone
  • Hydrogen peroxide
  • Oxidative stress
  • ROS

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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