Photooxidation of skeletal muscle sarcoplasmic reticulum induces rapid calcium release

Janice Stuart, Isaac N Pessah, Terence G. Favero, Jonathan J. Abramson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The photooxidizing xanthene dye rose bengal is shown to induce rapid Ca2+ release from skeletal muscle sarcoplasmic reticulum (SR) vesicles. In the presence of light, nanomolar concentrations of rose bengal increase the Ca2+ permeability of the SR and stimulate the production of singlet oxygen (1O2). In the absence of light, no 1O2 production is measured. Under these conditions, higher concentrations of rose bengal (micromolar) are required to stimulate Ca2+ release. Furthermore, removal of oxygen from the release medium results in marked inhibition of the light-dependent reaction rate. Rose bengal-induced Ca2+ release is relatively insensitive to Mg2+. At nanomolar concentrations, rose bengal inhibits [3H]ryanodine binding to its receptor. β,γ-Methyleneadenosine 5′-triphosphate, a nonhydrolyzable analog of ATP, inhibits rose bengal-induced Ca2+ release and prevents rose bengal inhibition of [3H]ryanodine binding. Ethoxyformic anhydride, a histidine modifying reagent, at millimolar concentrations induces Ca2+ release from SR vesicles in a manner similar to that of rose bengal. The molecular mechanism underlying rose bengal modification of the Ca2+ release system of the SR appears to involve a modification of a histidyl residue associated with the Ca2+ release protein from SR. The light-depedent reaction appears to be mediated by singlet oxygen.

Original languageEnglish (US)
Pages (from-to)512-521
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume292
Issue number2
DOIs
StatePublished - Feb 1 1992
Externally publishedYes

Fingerprint

Rose Bengal
Photooxidation
Sarcoplasmic Reticulum
Muscle
Skeletal Muscle
Calcium
Light
Ryanodine
Singlet Oxygen
Xanthenes
Diethyl Pyrocarbonate
Histidine
Reaction rates
Permeability
Coloring Agents
Adenosine Triphosphate
Oxygen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Photooxidation of skeletal muscle sarcoplasmic reticulum induces rapid calcium release. / Stuart, Janice; Pessah, Isaac N; Favero, Terence G.; Abramson, Jonathan J.

In: Archives of Biochemistry and Biophysics, Vol. 292, No. 2, 01.02.1992, p. 512-521.

Research output: Contribution to journalArticle

Stuart, Janice ; Pessah, Isaac N ; Favero, Terence G. ; Abramson, Jonathan J. / Photooxidation of skeletal muscle sarcoplasmic reticulum induces rapid calcium release. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 292, No. 2. pp. 512-521.
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