Photodynamic inactivation of an RNA enveloped virus in goat colostrum

K. E. Washburn, R. N. Streeter, J. T. Saliki, Terry W Lehenbauer, M. E. Prado

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The primary objective of this study was to determine if a RNA enveloped virus can be inactivated in goat colostrum using methylene blue (MB) and 1 h of illumination (photodynamic inactivation (PI)). The effects of illumination on the virucidal properties of MB were studied in translucent media as well as colostrum. Concentrations of MB evaluated were 10, 1.0, 0.1 and 0.01 μM. Bovine viral diarrhea virus (BVDV) was used as a model for caprine arthritis-encephalitis virus (CAEV). Virus, immunoglobulin, bacterial and coliform levels were determined before and after treatments. In transparent media, concentrations of MB >0.1 μM inactivated virus regardless of time, with or without light exposure. Sixty minutes of light treatment significantly reduced viral levels in samples containing 0.01 μM MB as compared to dark treatment. In colostrum, light treatment significantly inactivated virus in samples containing 1.0 and 10 μM MB. Concentrations of immunoglobulin were not adversely affected, and total bacterial and coliform counts increased. We concluded that PI of a RNA enveloped virus appears to be achievable in goat colostrum without lowering immunoglobulin concentrations, or creating a hazardous degree of bacterial and coliform proliferation.The clinical relevance of this experiment is that PI has the potential to inactivate CAEV in goat colostrum, thus providing the goat industry an alternative to heat treatment of colostrum. However, further refinement of this technique is warranted.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalSmall Ruminant Research
Volume42
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Colostrum
methylene blue
Methylene Blue
RNA Viruses
colostrum
Goats
inactivation
goats
Caprine Arthritis-Encephalitis Viruses
Caprine arthritis encephalitis virus
immunoglobulins
Immunoglobulins
Viruses
Lighting
Light
viruses
lighting
Bovine Viral Diarrhea Viruses
Bacterial Load
Bovine viral diarrhea virus

Keywords

  • Colostrum
  • Goat
  • Photodynamic inactivation
  • Virus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Photodynamic inactivation of an RNA enveloped virus in goat colostrum. / Washburn, K. E.; Streeter, R. N.; Saliki, J. T.; Lehenbauer, Terry W; Prado, M. E.

In: Small Ruminant Research, Vol. 42, No. 1, 2001, p. 31-37.

Research output: Contribution to journalArticle

Washburn, K. E. ; Streeter, R. N. ; Saliki, J. T. ; Lehenbauer, Terry W ; Prado, M. E. / Photodynamic inactivation of an RNA enveloped virus in goat colostrum. In: Small Ruminant Research. 2001 ; Vol. 42, No. 1. pp. 31-37.
@article{2314aeda399346cc8a767080f19b6b28,
title = "Photodynamic inactivation of an RNA enveloped virus in goat colostrum",
abstract = "The primary objective of this study was to determine if a RNA enveloped virus can be inactivated in goat colostrum using methylene blue (MB) and 1 h of illumination (photodynamic inactivation (PI)). The effects of illumination on the virucidal properties of MB were studied in translucent media as well as colostrum. Concentrations of MB evaluated were 10, 1.0, 0.1 and 0.01 μM. Bovine viral diarrhea virus (BVDV) was used as a model for caprine arthritis-encephalitis virus (CAEV). Virus, immunoglobulin, bacterial and coliform levels were determined before and after treatments. In transparent media, concentrations of MB >0.1 μM inactivated virus regardless of time, with or without light exposure. Sixty minutes of light treatment significantly reduced viral levels in samples containing 0.01 μM MB as compared to dark treatment. In colostrum, light treatment significantly inactivated virus in samples containing 1.0 and 10 μM MB. Concentrations of immunoglobulin were not adversely affected, and total bacterial and coliform counts increased. We concluded that PI of a RNA enveloped virus appears to be achievable in goat colostrum without lowering immunoglobulin concentrations, or creating a hazardous degree of bacterial and coliform proliferation.The clinical relevance of this experiment is that PI has the potential to inactivate CAEV in goat colostrum, thus providing the goat industry an alternative to heat treatment of colostrum. However, further refinement of this technique is warranted.",
keywords = "Colostrum, Goat, Photodynamic inactivation, Virus",
author = "Washburn, {K. E.} and Streeter, {R. N.} and Saliki, {J. T.} and Lehenbauer, {Terry W} and Prado, {M. E.}",
year = "2001",
doi = "10.1016/S0921-4488(01)00230-9",
language = "English (US)",
volume = "42",
pages = "31--37",
journal = "Small Ruminant Research",
issn = "0921-4488",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Photodynamic inactivation of an RNA enveloped virus in goat colostrum

AU - Washburn, K. E.

AU - Streeter, R. N.

AU - Saliki, J. T.

AU - Lehenbauer, Terry W

AU - Prado, M. E.

PY - 2001

Y1 - 2001

N2 - The primary objective of this study was to determine if a RNA enveloped virus can be inactivated in goat colostrum using methylene blue (MB) and 1 h of illumination (photodynamic inactivation (PI)). The effects of illumination on the virucidal properties of MB were studied in translucent media as well as colostrum. Concentrations of MB evaluated were 10, 1.0, 0.1 and 0.01 μM. Bovine viral diarrhea virus (BVDV) was used as a model for caprine arthritis-encephalitis virus (CAEV). Virus, immunoglobulin, bacterial and coliform levels were determined before and after treatments. In transparent media, concentrations of MB >0.1 μM inactivated virus regardless of time, with or without light exposure. Sixty minutes of light treatment significantly reduced viral levels in samples containing 0.01 μM MB as compared to dark treatment. In colostrum, light treatment significantly inactivated virus in samples containing 1.0 and 10 μM MB. Concentrations of immunoglobulin were not adversely affected, and total bacterial and coliform counts increased. We concluded that PI of a RNA enveloped virus appears to be achievable in goat colostrum without lowering immunoglobulin concentrations, or creating a hazardous degree of bacterial and coliform proliferation.The clinical relevance of this experiment is that PI has the potential to inactivate CAEV in goat colostrum, thus providing the goat industry an alternative to heat treatment of colostrum. However, further refinement of this technique is warranted.

AB - The primary objective of this study was to determine if a RNA enveloped virus can be inactivated in goat colostrum using methylene blue (MB) and 1 h of illumination (photodynamic inactivation (PI)). The effects of illumination on the virucidal properties of MB were studied in translucent media as well as colostrum. Concentrations of MB evaluated were 10, 1.0, 0.1 and 0.01 μM. Bovine viral diarrhea virus (BVDV) was used as a model for caprine arthritis-encephalitis virus (CAEV). Virus, immunoglobulin, bacterial and coliform levels were determined before and after treatments. In transparent media, concentrations of MB >0.1 μM inactivated virus regardless of time, with or without light exposure. Sixty minutes of light treatment significantly reduced viral levels in samples containing 0.01 μM MB as compared to dark treatment. In colostrum, light treatment significantly inactivated virus in samples containing 1.0 and 10 μM MB. Concentrations of immunoglobulin were not adversely affected, and total bacterial and coliform counts increased. We concluded that PI of a RNA enveloped virus appears to be achievable in goat colostrum without lowering immunoglobulin concentrations, or creating a hazardous degree of bacterial and coliform proliferation.The clinical relevance of this experiment is that PI has the potential to inactivate CAEV in goat colostrum, thus providing the goat industry an alternative to heat treatment of colostrum. However, further refinement of this technique is warranted.

KW - Colostrum

KW - Goat

KW - Photodynamic inactivation

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=0034814418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034814418&partnerID=8YFLogxK

U2 - 10.1016/S0921-4488(01)00230-9

DO - 10.1016/S0921-4488(01)00230-9

M3 - Article

VL - 42

SP - 31

EP - 37

JO - Small Ruminant Research

JF - Small Ruminant Research

SN - 0921-4488

IS - 1

ER -