Photodegradable hydrogels for capture, detection, and release of live cells

Dong Sik Shin, Jungmok You, Ali Rahimian, Tam Vu, Christian Siltanen, Arshia Ehsanipour, Gulnaz Stybayeva, Julie Sutcliffe, Alexander Revzin

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95 % purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-α antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence "halo" after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.

Original languageEnglish (US)
Pages (from-to)8221-8224
Number of pages4
JournalAngewandte Chemie - International Edition
Volume53
Issue number31
DOIs
StatePublished - Jul 28 2014

Keywords

  • antibodies
  • hydrogels
  • microarrays
  • micropatterning
  • single-cell detection

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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    Shin, D. S., You, J., Rahimian, A., Vu, T., Siltanen, C., Ehsanipour, A., Stybayeva, G., Sutcliffe, J., & Revzin, A. (2014). Photodegradable hydrogels for capture, detection, and release of live cells. Angewandte Chemie - International Edition, 53(31), 8221-8224. https://doi.org/10.1002/anie.201404323