Abstract
A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1145-1151 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 133 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 31 1985 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Photo-induced nicking of deoxyribonucleic acid by ruthenium(II)-bleomycin in the presence of air. / Subramanian, Ramaswamy; Meares, Claude F.
In: Biochemical and Biophysical Research Communications, Vol. 133, No. 3, 31.12.1985, p. 1145-1151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photo-induced nicking of deoxyribonucleic acid by ruthenium(II)-bleomycin in the presence of air
AU - Subramanian, Ramaswamy
AU - Meares, Claude F.
PY - 1985/12/31
Y1 - 1985/12/31
N2 - A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
AB - A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022297931&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(85)91256-2
DO - 10.1016/0006-291X(85)91256-2
M3 - Article
C2 - 2417601
AN - SCOPUS:0022297931
VL - 133
SP - 1145
EP - 1151
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -