A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 31 1985|
ASJC Scopus subject areas
- Molecular Biology