Photo-induced nicking of deoxyribonucleic acid by ruthenium(II)-bleomycin in the presence of air

Ramaswamy Subramanian, Claude F. Meares

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.

Original languageEnglish (US)
Pages (from-to)1145-1151
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number3
DOIs
StatePublished - Dec 31 1985

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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