Abstract
A new metallobleomycin, ruthenium(II)-blemycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 μM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3′-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redoxmediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1145-1151 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 133 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 31 1985 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Cite this
Subramanian, R., & Meares, C. F. (1985). Photo-induced nicking of deoxyribonucleic acid by ruthenium(II)-bleomycin in the presence of air. Biochemical and Biophysical Research Communications, 133(3), 1145-1151. https://doi.org/10.1016/0006-291X(85)91256-2