Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast

Frédérique Gaits, Geneviève Degols, Kazuhiro Shiozaki, Paul Russell

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Control of gene expression by stress-activated protein kinase (SAPK) cascades is crucial for combating cytotoxic stress. Elements of these cascades have been investigated in detail, but regulation of stress signal transduction from the cytoplasm to the nucleus is poorly understood. Herein are reported subcellular localization studies of fission yeast Spc1, a homolog of human p38 and budding yeast Hog1p SAPKs. Stress induces transient nuclear localization of Spc1. Nuclear translocation of Spc1 is coupled with disassociation from its activator kinase Wis1. However, Spc1 does not concentrate in the nucleus of δwis1 cells; therefore Wis1 does not tether Spc1 in the cytoplasm. Unphosphorylatable forms of Spc1 are dispersed in the cytoplasm and nucleus, even in cells that also produce wild-type Spc1. Thus, Spc1 must be phosphorylated by Wis1 to localize in the nucleus. Nuclear retention of Spc1 requires Atf1, a transcription factor that is the key nuclear substrate of Spc1. Nuclear localization of Atf1 requires Pcr1, a heterodimerization partner of Atf1. These studies show that phosphorylation and association with Atf1 are required for nuclear localization of Spc1.

Original languageEnglish (US)
Pages (from-to)1464-1473
Number of pages10
JournalGenes and Development
Volume12
Issue number10
StatePublished - May 15 1998

Fingerprint

Schizosaccharomyces
Cytoplasm
Transcription Factors
Phosphotransferases
Phosphorylation
Saccharomycetales
Heat-Shock Proteins
Cell Nucleus
Protein Kinases
Signal Transduction
Gene Expression

Keywords

  • Atf1
  • Fission yeast
  • Nuclear localization
  • Schizosaccharomyces pombe
  • Spc1
  • Stress-activated protein kinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast. / Gaits, Frédérique; Degols, Geneviève; Shiozaki, Kazuhiro; Russell, Paul.

In: Genes and Development, Vol. 12, No. 10, 15.05.1998, p. 1464-1473.

Research output: Contribution to journalArticle

Gaits, Frédérique ; Degols, Geneviève ; Shiozaki, Kazuhiro ; Russell, Paul. / Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast. In: Genes and Development. 1998 ; Vol. 12, No. 10. pp. 1464-1473.
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