Abstract
Using monoclonal antibody (Ta-51) that specifically binds phosphorylated high molecular weight neurofilament (pNFH) proteins, we investigated the occurrence of perikaryal pNFH in the spinal ventral horn motoneurons, intermediolateral column (ILC) neurons, sympathetic ganglion neurons and dorsal root ganglion (DRG) neurons obtained from patients with amyotrophic lateral sclerosis (ALS) and multiple system atrophy (MSA) and from control cases. In the controls, a system-dependent variation in perikaryal Ta-51 immunoreactivity was observed. Very few ventral horn cells and ILC neurons were stained with Ta-51, while large population of DRG neurons and sympathetic neurons were Ta-51 positive. The incidence of perikaryal immunoreactivity in the ventral horn cells was significantly increased in ALS and MSA. Some ILC neurons in ALS were Ta-51 positive and their incidence was significantly higher than that of the controls. These data suggest that both ILC neurons and ventral horn cells are affected with respect to pNFH metabolism in ALS and MSA. No significant difference was, however, detected in the Ta-51 immunoreactivity of both DRG and sympathetic ganglion neurons in ALS and MSA as compared with the controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 240-245 |
| Number of pages | 6 |
| Journal | Acta Neuropathologica |
| Volume | 83 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 1992 |
| Externally published | Yes |
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Keywords
- Amyotrophic lateral sclerosis
- High molecular weight neurofilament
- Multiple system atrophy
- Peripheral nervous system
- Phosphorylation
ASJC Scopus subject areas
- Neuroscience(all)
- Pathology and Forensic Medicine
- Clinical Neurology
Cite this
Phosphorylated high molecular weight neurofilament protein in the peripheral motor, sensory and sympathetic neuronal perikarya : system-dependent normal variations and changes in amyotrophic lateral sclerosis and multiple system atrophy. / Ito, Takayuki; Sobue, G.; Ken, E.; Mitsuma, T.; Takahashi, A.; Trojanowski, J. Q.
In: Acta Neuropathologica, Vol. 83, No. 3, 02.1992, p. 240-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorylated high molecular weight neurofilament protein in the peripheral motor, sensory and sympathetic neuronal perikarya
T2 - system-dependent normal variations and changes in amyotrophic lateral sclerosis and multiple system atrophy
AU - Ito, Takayuki
AU - Sobue, G.
AU - Ken, E.
AU - Mitsuma, T.
AU - Takahashi, A.
AU - Trojanowski, J. Q.
PY - 1992/2
Y1 - 1992/2
N2 - Using monoclonal antibody (Ta-51) that specifically binds phosphorylated high molecular weight neurofilament (pNFH) proteins, we investigated the occurrence of perikaryal pNFH in the spinal ventral horn motoneurons, intermediolateral column (ILC) neurons, sympathetic ganglion neurons and dorsal root ganglion (DRG) neurons obtained from patients with amyotrophic lateral sclerosis (ALS) and multiple system atrophy (MSA) and from control cases. In the controls, a system-dependent variation in perikaryal Ta-51 immunoreactivity was observed. Very few ventral horn cells and ILC neurons were stained with Ta-51, while large population of DRG neurons and sympathetic neurons were Ta-51 positive. The incidence of perikaryal immunoreactivity in the ventral horn cells was significantly increased in ALS and MSA. Some ILC neurons in ALS were Ta-51 positive and their incidence was significantly higher than that of the controls. These data suggest that both ILC neurons and ventral horn cells are affected with respect to pNFH metabolism in ALS and MSA. No significant difference was, however, detected in the Ta-51 immunoreactivity of both DRG and sympathetic ganglion neurons in ALS and MSA as compared with the controls.
AB - Using monoclonal antibody (Ta-51) that specifically binds phosphorylated high molecular weight neurofilament (pNFH) proteins, we investigated the occurrence of perikaryal pNFH in the spinal ventral horn motoneurons, intermediolateral column (ILC) neurons, sympathetic ganglion neurons and dorsal root ganglion (DRG) neurons obtained from patients with amyotrophic lateral sclerosis (ALS) and multiple system atrophy (MSA) and from control cases. In the controls, a system-dependent variation in perikaryal Ta-51 immunoreactivity was observed. Very few ventral horn cells and ILC neurons were stained with Ta-51, while large population of DRG neurons and sympathetic neurons were Ta-51 positive. The incidence of perikaryal immunoreactivity in the ventral horn cells was significantly increased in ALS and MSA. Some ILC neurons in ALS were Ta-51 positive and their incidence was significantly higher than that of the controls. These data suggest that both ILC neurons and ventral horn cells are affected with respect to pNFH metabolism in ALS and MSA. No significant difference was, however, detected in the Ta-51 immunoreactivity of both DRG and sympathetic ganglion neurons in ALS and MSA as compared with the controls.
KW - Amyotrophic lateral sclerosis
KW - High molecular weight neurofilament
KW - Multiple system atrophy
KW - Peripheral nervous system
KW - Phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=0026499998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026499998&partnerID=8YFLogxK
U2 - 10.1007/BF00296785
DO - 10.1007/BF00296785
M3 - Article
C2 - 1557955
AN - SCOPUS:0026499998
VL - 83
SP - 240
EP - 245
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 3
ER -