Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion

Lorenzo Rossaro, Noriko Murase, Cary Caldwell, Hassan Farghali, Adrian Casavilla, Thomas E. Starzl, Chien Ho, David H. van Thiel

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The adenosine triphosphate (ATP) content and intracellular pH (pH1) of isolated rat liver before, during, and after cold preservation in either University of Wisconsin lactobionate solution (UW) (n = 10) or Euro-Collins solution (EC), (n = 8) were monitored with phosphorus 31 nuclear magnetic resonance. The 31P nuclear magnetic resonance spectra were obtained on a 4.7 T system operating at 81 MHz. Fructose metabolism, liver enzyme release, oxygen consumption, and rat survival after liver transplantation were also evaluated. During simple cold storage (SCS) the ATP level declined to undetectable levels with both preservation solutions whereas the pH1 declined to approximately 7.0. In contrast, during continuous hypothermic perfusion (CHP), hepatic ATP levels remained measurable during the 24-hour EC preservation and actually increased significantly (p > 0.01) during UW preservation. After reperfusion at 37 °C with Krebs lactate, the livers in SCS treated with EC differed significantly from the UW-treated livers in terms of their ATP level and pH1 and their response to a fructose challenge. In contrast, livers undergoing CHP demonstrated similar behaviors with both solutions. These results demonstrate an increase in the hepatic ATP content during CHP, which occurs with UW but is not seen with EC. On the other hand, only livers that were simply stored with UW achieved significant survival after transplantation, whereas CHP livers were affected by vascular damage as demonstrated by fatal thrombosis after transplantation. These data suggest that ATP content is not the only determinant of good liver function. A system of hypothermic perfusion might further improve liver preservation efficacy should injury to the vascular endothelium be avoided.

Original languageEnglish (US)
Pages (from-to)559-568
Number of pages10
JournalThe Journal of Laboratory and Clinical Medicine
Volume120
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Liver
Phosphorus
Nuclear magnetic resonance spectroscopy
Rats
Magnetic Resonance Spectroscopy
Perfusion
Adenosine Triphosphate
Cold storage
Fructose
Nuclear magnetic resonance
Transplantation
Vascular Endothelium
Metabolism
Oxygen Consumption
Liver Transplantation
Lactic Acid
Reperfusion
Blood Vessels
Thrombosis
Oxygen

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Rossaro, L., Murase, N., Caldwell, C., Farghali, H., Casavilla, A., Starzl, T. E., ... van Thiel, D. H. (1992). Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. The Journal of Laboratory and Clinical Medicine, 120(4), 559-568.

Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. / Rossaro, Lorenzo; Murase, Noriko; Caldwell, Cary; Farghali, Hassan; Casavilla, Adrian; Starzl, Thomas E.; Ho, Chien; van Thiel, David H.

In: The Journal of Laboratory and Clinical Medicine, Vol. 120, No. 4, 1992, p. 559-568.

Research output: Contribution to journalArticle

Rossaro, L, Murase, N, Caldwell, C, Farghali, H, Casavilla, A, Starzl, TE, Ho, C & van Thiel, DH 1992, 'Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion', The Journal of Laboratory and Clinical Medicine, vol. 120, no. 4, pp. 559-568.
Rossaro, Lorenzo ; Murase, Noriko ; Caldwell, Cary ; Farghali, Hassan ; Casavilla, Adrian ; Starzl, Thomas E. ; Ho, Chien ; van Thiel, David H. / Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. In: The Journal of Laboratory and Clinical Medicine. 1992 ; Vol. 120, No. 4. pp. 559-568.
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