Abstract
The adenosine triphosphate (ATP) content and intracellular pH (pH1) of isolated rat liver before, during, and after cold preservation in either University of Wisconsin lactobionate solution (UW) (n = 10) or Euro-Collins solution (EC), (n = 8) were monitored with phosphorus 31 nuclear magnetic resonance. The 31P nuclear magnetic resonance spectra were obtained on a 4.7 T system operating at 81 MHz. Fructose metabolism, liver enzyme release, oxygen consumption, and rat survival after liver transplantation were also evaluated. During simple cold storage (SCS) the ATP level declined to undetectable levels with both preservation solutions whereas the pH1 declined to approximately 7.0. In contrast, during continuous hypothermic perfusion (CHP), hepatic ATP levels remained measurable during the 24-hour EC preservation and actually increased significantly (p > 0.01) during UW preservation. After reperfusion at 37 °C with Krebs lactate, the livers in SCS treated with EC differed significantly from the UW-treated livers in terms of their ATP level and pH1 and their response to a fructose challenge. In contrast, livers undergoing CHP demonstrated similar behaviors with both solutions. These results demonstrate an increase in the hepatic ATP content during CHP, which occurs with UW but is not seen with EC. On the other hand, only livers that were simply stored with UW achieved significant survival after transplantation, whereas CHP livers were affected by vascular damage as demonstrated by fatal thrombosis after transplantation. These data suggest that ATP content is not the only determinant of good liver function. A system of hypothermic perfusion might further improve liver preservation efficacy should injury to the vascular endothelium be avoided.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 559-568 |
| Number of pages | 10 |
| Journal | The Journal of Laboratory and Clinical Medicine |
| Volume | 120 |
| Issue number | 4 |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
- Pathology and Forensic Medicine
Cite this
Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. / Rossaro, Lorenzo; Murase, Noriko; Caldwell, Cary; Farghali, Hassan; Casavilla, Adrian; Starzl, Thomas E.; Ho, Chien; van Thiel, David H.
In: The Journal of Laboratory and Clinical Medicine, Vol. 120, No. 4, 1992, p. 559-568.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion
AU - Rossaro, Lorenzo
AU - Murase, Noriko
AU - Caldwell, Cary
AU - Farghali, Hassan
AU - Casavilla, Adrian
AU - Starzl, Thomas E.
AU - Ho, Chien
AU - van Thiel, David H.
PY - 1992
Y1 - 1992
N2 - The adenosine triphosphate (ATP) content and intracellular pH (pH1) of isolated rat liver before, during, and after cold preservation in either University of Wisconsin lactobionate solution (UW) (n = 10) or Euro-Collins solution (EC), (n = 8) were monitored with phosphorus 31 nuclear magnetic resonance. The 31P nuclear magnetic resonance spectra were obtained on a 4.7 T system operating at 81 MHz. Fructose metabolism, liver enzyme release, oxygen consumption, and rat survival after liver transplantation were also evaluated. During simple cold storage (SCS) the ATP level declined to undetectable levels with both preservation solutions whereas the pH1 declined to approximately 7.0. In contrast, during continuous hypothermic perfusion (CHP), hepatic ATP levels remained measurable during the 24-hour EC preservation and actually increased significantly (p > 0.01) during UW preservation. After reperfusion at 37 °C with Krebs lactate, the livers in SCS treated with EC differed significantly from the UW-treated livers in terms of their ATP level and pH1 and their response to a fructose challenge. In contrast, livers undergoing CHP demonstrated similar behaviors with both solutions. These results demonstrate an increase in the hepatic ATP content during CHP, which occurs with UW but is not seen with EC. On the other hand, only livers that were simply stored with UW achieved significant survival after transplantation, whereas CHP livers were affected by vascular damage as demonstrated by fatal thrombosis after transplantation. These data suggest that ATP content is not the only determinant of good liver function. A system of hypothermic perfusion might further improve liver preservation efficacy should injury to the vascular endothelium be avoided.
AB - The adenosine triphosphate (ATP) content and intracellular pH (pH1) of isolated rat liver before, during, and after cold preservation in either University of Wisconsin lactobionate solution (UW) (n = 10) or Euro-Collins solution (EC), (n = 8) were monitored with phosphorus 31 nuclear magnetic resonance. The 31P nuclear magnetic resonance spectra were obtained on a 4.7 T system operating at 81 MHz. Fructose metabolism, liver enzyme release, oxygen consumption, and rat survival after liver transplantation were also evaluated. During simple cold storage (SCS) the ATP level declined to undetectable levels with both preservation solutions whereas the pH1 declined to approximately 7.0. In contrast, during continuous hypothermic perfusion (CHP), hepatic ATP levels remained measurable during the 24-hour EC preservation and actually increased significantly (p > 0.01) during UW preservation. After reperfusion at 37 °C with Krebs lactate, the livers in SCS treated with EC differed significantly from the UW-treated livers in terms of their ATP level and pH1 and their response to a fructose challenge. In contrast, livers undergoing CHP demonstrated similar behaviors with both solutions. These results demonstrate an increase in the hepatic ATP content during CHP, which occurs with UW but is not seen with EC. On the other hand, only livers that were simply stored with UW achieved significant survival after transplantation, whereas CHP livers were affected by vascular damage as demonstrated by fatal thrombosis after transplantation. These data suggest that ATP content is not the only determinant of good liver function. A system of hypothermic perfusion might further improve liver preservation efficacy should injury to the vascular endothelium be avoided.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026448307&partnerID=8YFLogxK
M3 - Article
VL - 120
SP - 559
EP - 568
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 4
ER -