Phosphorelay-dependent and-independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast

Susumu Morigasaki, Kazuhiro Shiozaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In a "two-component system," extracellular stimuli are transmitted by the transfer of a phosphoryl group from a sensor histidine kinase to a response regulator (RR), a mechanism referred to as phosphorelay. In the fission yeast Schizosaccharomyces pombe, peroxide stress signals are transmitted by phosphorelay to the Mcs4 RR, which activates the Spc1 MAP kinase (MAPK) cascade. We previously demonstrated that a glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) physically interacts with Mcs4 and promotes phosphorelay signaling to Mcs4. Independently of the phosphorelay mechanism, Mcs4 also plays a critical role in osmostress signaling, as a part of the stable ternary complex with the Wis4 and Win1 MAPK kinase kinases (MAPKKKs). Interestingly, GAPDH dissociates from Mcs4 upon osmostress, while oxidative stress promotes their association. The Mcs4 RR may serve as a switching hub that mediates activation of the Wis4-Win1 MAPKKK heteromer in response to different forms of stress.

Original languageEnglish (US)
Article numbere25020
JournalCommunicative and Integrative Biology
Volume6
Issue number5
DOIs
StatePublished - 2013

Fingerprint

mitogen-activated protein kinase kinase
Glyceraldehyde-3-Phosphate Dehydrogenases
glyceraldehyde-3-phosphate dehydrogenase
Schizosaccharomyces
Schizosaccharomyces pombe
Mitogen-Activated Protein Kinase Kinases
phosphotransferases (kinases)
Phosphotransferases
mitogen-activated protein kinase kinase kinase
MAP Kinase Kinase Kinases
histidine kinase
MAP Kinase Signaling System
Peroxides
peroxides
mitogen-activated protein kinase
Oxidative Stress
oxidative stress
Enzymes
enzymes
Histidine Kinase

Keywords

  • Glyceraldehyde-3-phosphate dehydrogenase
  • Response regulator
  • Signal transduction
  • Stress response
  • Stress-activated protein kinase
  • Two-component system

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Phosphorelay-dependent and-independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast. / Morigasaki, Susumu; Shiozaki, Kazuhiro.

In: Communicative and Integrative Biology, Vol. 6, No. 5, e25020, 2013.

Research output: Contribution to journalArticle

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