Phospholipid vesicles increase the survival of freeze-dried human red blood cells

Azadeh Kheirolomoom, Gyana R. Satpathy, Zsolt Török, Mitali Banerjee, Rachna Bali, Roberta C. Novaes, Erika Little, Danielle M. Manning, Denis M Dwyre, Fern Tablin, John H. Crowe, Nelly M. Tsvetkova

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

In a previous report [Z. Török, G. Satpathy, M. Banerjee, R. Bali, E. Little, R. Novaes, H. Van Ly, D. Dwyre, A. Kheirolomoom, F. Tablin, J.H. Crowe, N.M. Tsvetkova, Preservation of trehalose loaded red blood cells by lyophilization, Cell Preservation Technol. 3 (2005) 96-111.], we presented a method for preserving human red blood cells (RBCs) by loading them with trehalose and then freeze-drying. We have now improved that method, based on the discovery that addition of phospholipid vesicles to the lyophilization buffer substantially reduces hemolysis of freeze-dried RBCs after rehydration. The surviving cells synthesize 2,3-DPG, have low levels of methemoglobin, and have preserved morphology. Among the lipid species we studied, unsaturated PCs were found to be most effective in suppressing hemoglobin leakage. RBC-vesicle interactions depend on vesicle size and structure; unilamellar liposomes with average diameter of less than 300 nm were more effective in reducing the hemolysis than multilamellar vesicles. Trehalose loaded RBCs demonstrated high survival and low levels of methemoglobin during 10 weeks of storage at 4°C in the dry state when lyophilized in the presence of liposomes.

Original languageEnglish (US)
Pages (from-to)290-305
Number of pages16
JournalCryobiology
Volume51
Issue number3
DOIs
StatePublished - Dec 2005

Keywords

  • Erythrocytes
  • Freeze-drying
  • Liposomes
  • Long-term stability
  • Preservation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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    Kheirolomoom, A., Satpathy, G. R., Török, Z., Banerjee, M., Bali, R., Novaes, R. C., Little, E., Manning, D. M., Dwyre, D. M., Tablin, F., Crowe, J. H., & Tsvetkova, N. M. (2005). Phospholipid vesicles increase the survival of freeze-dried human red blood cells. Cryobiology, 51(3), 290-305. https://doi.org/10.1016/j.cryobiol.2005.08.003