Phospholipid morphologies on photochemically patterned silane monolayers

Michael C. Howland, Annapoorna R. Sapuri-Butti, Sanhita S. Dixit, Andrew M. Dattelbaum, Andrew P. Shreve, Atul N. Parikh

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We have studied the spreading of phospholipid vesicles on photochemically patterned n-octadecylsiloxane monolayers using epifluorescence and imaging ellipsometry measurements. Self-assembled monolayers of n-octadecylsiloxanes were patterned using short-wavelength ultraviolet radiation and a photomask to produce periodic arrays of patterned hydrophilic domains separated from hydrophobic surroundings. Exposing these patterned surfaces to a solution of small unilamellar vesicles of phospholipids and their mixtures resulted in a complex lipid layer morphology epitaxially reflecting the underlying pattern of hydrophilicity. The hydrophilic square regions of the photopatterned OTS monolayer reflected lipid bilayer formation, and the hydrophobic OTS residues supported lipid monolayers. We further observed the existence of a boundary region composed of a nonfluid lipid phase and a lipid-free moat at the interface between the lipid monolayer and bilayer morphologies spontaneously corralling the fluid bilayers. The outer-edge of the boundary region was found to be accessible for subsequent adsorption by proteins (e.g., streptavidin and BSA), but the inner-edge closer to the bilayer remained resistant to adsorption by protein or vesicles. Mechanistic implications of our results in terms of the effects of substrate topochemical character are discussed. Furthermore, our results provide a basis for the construction of complex biomembrane models, which exhibit fluidity barriers and differentiate membrane properties based on correspondence between lipid leaflets. We also envisage the use of this construct where two-dimensionally fluid, low-defect lipid layers serve as sacrificial resists for the deposition of protein and other material patterns.

Original languageEnglish (US)
Pages (from-to)6752-6765
Number of pages14
JournalJournal of the American Chemical Society
Volume127
Issue number18
DOIs
StatePublished - May 11 2005
Externally publishedYes

Fingerprint

Silanes
Phospholipids
Lipids
Monolayers
Lipid Bilayers
Adsorption
Proteins
Unilamellar Liposomes
Streptavidin
Photomasks
Lipid bilayers
Fluids
Fluidity
Hydrophobic and Hydrophilic Interactions
Ellipsometry
Hydrophilicity
Self assembled monolayers
Ultraviolet radiation
Radiation
Membranes

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Howland, M. C., Sapuri-Butti, A. R., Dixit, S. S., Dattelbaum, A. M., Shreve, A. P., & Parikh, A. N. (2005). Phospholipid morphologies on photochemically patterned silane monolayers. Journal of the American Chemical Society, 127(18), 6752-6765. https://doi.org/10.1021/ja043439q

Phospholipid morphologies on photochemically patterned silane monolayers. / Howland, Michael C.; Sapuri-Butti, Annapoorna R.; Dixit, Sanhita S.; Dattelbaum, Andrew M.; Shreve, Andrew P.; Parikh, Atul N.

In: Journal of the American Chemical Society, Vol. 127, No. 18, 11.05.2005, p. 6752-6765.

Research output: Contribution to journalArticle

Howland, MC, Sapuri-Butti, AR, Dixit, SS, Dattelbaum, AM, Shreve, AP & Parikh, AN 2005, 'Phospholipid morphologies on photochemically patterned silane monolayers', Journal of the American Chemical Society, vol. 127, no. 18, pp. 6752-6765. https://doi.org/10.1021/ja043439q
Howland MC, Sapuri-Butti AR, Dixit SS, Dattelbaum AM, Shreve AP, Parikh AN. Phospholipid morphologies on photochemically patterned silane monolayers. Journal of the American Chemical Society. 2005 May 11;127(18):6752-6765. https://doi.org/10.1021/ja043439q
Howland, Michael C. ; Sapuri-Butti, Annapoorna R. ; Dixit, Sanhita S. ; Dattelbaum, Andrew M. ; Shreve, Andrew P. ; Parikh, Atul N. / Phospholipid morphologies on photochemically patterned silane monolayers. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 18. pp. 6752-6765.
@article{97be7819f06d488aaf90240cff808c5f,
title = "Phospholipid morphologies on photochemically patterned silane monolayers",
abstract = "We have studied the spreading of phospholipid vesicles on photochemically patterned n-octadecylsiloxane monolayers using epifluorescence and imaging ellipsometry measurements. Self-assembled monolayers of n-octadecylsiloxanes were patterned using short-wavelength ultraviolet radiation and a photomask to produce periodic arrays of patterned hydrophilic domains separated from hydrophobic surroundings. Exposing these patterned surfaces to a solution of small unilamellar vesicles of phospholipids and their mixtures resulted in a complex lipid layer morphology epitaxially reflecting the underlying pattern of hydrophilicity. The hydrophilic square regions of the photopatterned OTS monolayer reflected lipid bilayer formation, and the hydrophobic OTS residues supported lipid monolayers. We further observed the existence of a boundary region composed of a nonfluid lipid phase and a lipid-free moat at the interface between the lipid monolayer and bilayer morphologies spontaneously corralling the fluid bilayers. The outer-edge of the boundary region was found to be accessible for subsequent adsorption by proteins (e.g., streptavidin and BSA), but the inner-edge closer to the bilayer remained resistant to adsorption by protein or vesicles. Mechanistic implications of our results in terms of the effects of substrate topochemical character are discussed. Furthermore, our results provide a basis for the construction of complex biomembrane models, which exhibit fluidity barriers and differentiate membrane properties based on correspondence between lipid leaflets. We also envisage the use of this construct where two-dimensionally fluid, low-defect lipid layers serve as sacrificial resists for the deposition of protein and other material patterns.",
author = "Howland, {Michael C.} and Sapuri-Butti, {Annapoorna R.} and Dixit, {Sanhita S.} and Dattelbaum, {Andrew M.} and Shreve, {Andrew P.} and Parikh, {Atul N.}",
year = "2005",
month = "5",
day = "11",
doi = "10.1021/ja043439q",
language = "English (US)",
volume = "127",
pages = "6752--6765",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "18",

}

TY - JOUR

T1 - Phospholipid morphologies on photochemically patterned silane monolayers

AU - Howland, Michael C.

AU - Sapuri-Butti, Annapoorna R.

AU - Dixit, Sanhita S.

AU - Dattelbaum, Andrew M.

AU - Shreve, Andrew P.

AU - Parikh, Atul N.

PY - 2005/5/11

Y1 - 2005/5/11

N2 - We have studied the spreading of phospholipid vesicles on photochemically patterned n-octadecylsiloxane monolayers using epifluorescence and imaging ellipsometry measurements. Self-assembled monolayers of n-octadecylsiloxanes were patterned using short-wavelength ultraviolet radiation and a photomask to produce periodic arrays of patterned hydrophilic domains separated from hydrophobic surroundings. Exposing these patterned surfaces to a solution of small unilamellar vesicles of phospholipids and their mixtures resulted in a complex lipid layer morphology epitaxially reflecting the underlying pattern of hydrophilicity. The hydrophilic square regions of the photopatterned OTS monolayer reflected lipid bilayer formation, and the hydrophobic OTS residues supported lipid monolayers. We further observed the existence of a boundary region composed of a nonfluid lipid phase and a lipid-free moat at the interface between the lipid monolayer and bilayer morphologies spontaneously corralling the fluid bilayers. The outer-edge of the boundary region was found to be accessible for subsequent adsorption by proteins (e.g., streptavidin and BSA), but the inner-edge closer to the bilayer remained resistant to adsorption by protein or vesicles. Mechanistic implications of our results in terms of the effects of substrate topochemical character are discussed. Furthermore, our results provide a basis for the construction of complex biomembrane models, which exhibit fluidity barriers and differentiate membrane properties based on correspondence between lipid leaflets. We also envisage the use of this construct where two-dimensionally fluid, low-defect lipid layers serve as sacrificial resists for the deposition of protein and other material patterns.

AB - We have studied the spreading of phospholipid vesicles on photochemically patterned n-octadecylsiloxane monolayers using epifluorescence and imaging ellipsometry measurements. Self-assembled monolayers of n-octadecylsiloxanes were patterned using short-wavelength ultraviolet radiation and a photomask to produce periodic arrays of patterned hydrophilic domains separated from hydrophobic surroundings. Exposing these patterned surfaces to a solution of small unilamellar vesicles of phospholipids and their mixtures resulted in a complex lipid layer morphology epitaxially reflecting the underlying pattern of hydrophilicity. The hydrophilic square regions of the photopatterned OTS monolayer reflected lipid bilayer formation, and the hydrophobic OTS residues supported lipid monolayers. We further observed the existence of a boundary region composed of a nonfluid lipid phase and a lipid-free moat at the interface between the lipid monolayer and bilayer morphologies spontaneously corralling the fluid bilayers. The outer-edge of the boundary region was found to be accessible for subsequent adsorption by proteins (e.g., streptavidin and BSA), but the inner-edge closer to the bilayer remained resistant to adsorption by protein or vesicles. Mechanistic implications of our results in terms of the effects of substrate topochemical character are discussed. Furthermore, our results provide a basis for the construction of complex biomembrane models, which exhibit fluidity barriers and differentiate membrane properties based on correspondence between lipid leaflets. We also envisage the use of this construct where two-dimensionally fluid, low-defect lipid layers serve as sacrificial resists for the deposition of protein and other material patterns.

UR - http://www.scopus.com/inward/record.url?scp=18644376843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18644376843&partnerID=8YFLogxK

U2 - 10.1021/ja043439q

DO - 10.1021/ja043439q

M3 - Article

C2 - 15869298

AN - SCOPUS:18644376843

VL - 127

SP - 6752

EP - 6765

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 18

ER -