Phospholipid fatty acid composition of various mouse tissues after feeding α-linolenate (18: 3n-3) or eicosatrienoate (20:3n-3)

Alvin Berger, J. Bruce German

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The selective incorporation of dietary α-linolenate (18:3n-3) and its elongation product, eicosatrienoate (20:3n-3), into various phospholipids (PL) of mouse liver, spleen, kidney, and heart, was examined in a two-week feeding trial by assessing mol % changes in associated fatty acids. Mice were fed fat-free AIN 76A diets modified with either 2 wt% safflower oil (control); 1% safflower and 1% linolenate; or 1% safflower and 1% eicosatrienoate. After linolenate or eicosatrienoate feeding, 20:4n-6 was reduced by 36-50% in liver phosphatidylcholine (PC) and in liver and spleen phosphatidylethanolamine (PE). Linolenate was minimally incorporated into PL, but was desaturated and elongated to 20:5n-3, 22:5n-3, and 22:6n-3, with notable differences in the quantity of these n-3 derivatives associated with different tissues and PL. Eicosatrientoate was uniquely incorporated into the cardiolipin (CL) pool of all organs. There was also considerable retroconversion of 20:3n-3 to 18:3n-3 (PC, PE). Dietary eicosatrienoate may therefore affect metabolism in diverse ways-20:3n-3, which is retroconverted to 18:3n-3, may provide substrate for 20:5n-3 and 22:6n-3 syntheses, whereas intact 20:3n-3 may be incorporated into the CL pool. Acyl modifications of CL are known to affect the activity of key innermitochondrial enzymes, such as cytochrome c oxidase.

Original languageEnglish (US)
Pages (from-to)473-480
Number of pages8
JournalLipids
Volume25
Issue number8
DOIs
StatePublished - Aug 1990

Fingerprint

alpha-Linolenic Acid
cardiolipins
Cardiolipins
Phospholipids
phospholipids
Carthamus tinctorius
Fatty Acids
fatty acid composition
Liver
phosphatidylethanolamines
Tissue
phosphatidylcholines
liver
mice
spleen
Chemical analysis
Phosphatidylcholines
safflower oil
Spleen
Safflower Oil

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Organic Chemistry
  • Cell Biology

Cite this

Phospholipid fatty acid composition of various mouse tissues after feeding α-linolenate (18 : 3n-3) or eicosatrienoate (20:3n-3). / Berger, Alvin; German, J. Bruce.

In: Lipids, Vol. 25, No. 8, 08.1990, p. 473-480.

Research output: Contribution to journalArticle

@article{fa69b6d8be8e47ab8dc2599e08d14804,
title = "Phospholipid fatty acid composition of various mouse tissues after feeding α-linolenate (18: 3n-3) or eicosatrienoate (20:3n-3)",
abstract = "The selective incorporation of dietary α-linolenate (18:3n-3) and its elongation product, eicosatrienoate (20:3n-3), into various phospholipids (PL) of mouse liver, spleen, kidney, and heart, was examined in a two-week feeding trial by assessing mol {\%} changes in associated fatty acids. Mice were fed fat-free AIN 76A diets modified with either 2 wt{\%} safflower oil (control); 1{\%} safflower and 1{\%} linolenate; or 1{\%} safflower and 1{\%} eicosatrienoate. After linolenate or eicosatrienoate feeding, 20:4n-6 was reduced by 36-50{\%} in liver phosphatidylcholine (PC) and in liver and spleen phosphatidylethanolamine (PE). Linolenate was minimally incorporated into PL, but was desaturated and elongated to 20:5n-3, 22:5n-3, and 22:6n-3, with notable differences in the quantity of these n-3 derivatives associated with different tissues and PL. Eicosatrientoate was uniquely incorporated into the cardiolipin (CL) pool of all organs. There was also considerable retroconversion of 20:3n-3 to 18:3n-3 (PC, PE). Dietary eicosatrienoate may therefore affect metabolism in diverse ways-20:3n-3, which is retroconverted to 18:3n-3, may provide substrate for 20:5n-3 and 22:6n-3 syntheses, whereas intact 20:3n-3 may be incorporated into the CL pool. Acyl modifications of CL are known to affect the activity of key innermitochondrial enzymes, such as cytochrome c oxidase.",
author = "Alvin Berger and German, {J. Bruce}",
year = "1990",
month = "8",
doi = "10.1007/BF02538091",
language = "English (US)",
volume = "25",
pages = "473--480",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - Phospholipid fatty acid composition of various mouse tissues after feeding α-linolenate (18

T2 - 3n-3) or eicosatrienoate (20:3n-3)

AU - Berger, Alvin

AU - German, J. Bruce

PY - 1990/8

Y1 - 1990/8

N2 - The selective incorporation of dietary α-linolenate (18:3n-3) and its elongation product, eicosatrienoate (20:3n-3), into various phospholipids (PL) of mouse liver, spleen, kidney, and heart, was examined in a two-week feeding trial by assessing mol % changes in associated fatty acids. Mice were fed fat-free AIN 76A diets modified with either 2 wt% safflower oil (control); 1% safflower and 1% linolenate; or 1% safflower and 1% eicosatrienoate. After linolenate or eicosatrienoate feeding, 20:4n-6 was reduced by 36-50% in liver phosphatidylcholine (PC) and in liver and spleen phosphatidylethanolamine (PE). Linolenate was minimally incorporated into PL, but was desaturated and elongated to 20:5n-3, 22:5n-3, and 22:6n-3, with notable differences in the quantity of these n-3 derivatives associated with different tissues and PL. Eicosatrientoate was uniquely incorporated into the cardiolipin (CL) pool of all organs. There was also considerable retroconversion of 20:3n-3 to 18:3n-3 (PC, PE). Dietary eicosatrienoate may therefore affect metabolism in diverse ways-20:3n-3, which is retroconverted to 18:3n-3, may provide substrate for 20:5n-3 and 22:6n-3 syntheses, whereas intact 20:3n-3 may be incorporated into the CL pool. Acyl modifications of CL are known to affect the activity of key innermitochondrial enzymes, such as cytochrome c oxidase.

AB - The selective incorporation of dietary α-linolenate (18:3n-3) and its elongation product, eicosatrienoate (20:3n-3), into various phospholipids (PL) of mouse liver, spleen, kidney, and heart, was examined in a two-week feeding trial by assessing mol % changes in associated fatty acids. Mice were fed fat-free AIN 76A diets modified with either 2 wt% safflower oil (control); 1% safflower and 1% linolenate; or 1% safflower and 1% eicosatrienoate. After linolenate or eicosatrienoate feeding, 20:4n-6 was reduced by 36-50% in liver phosphatidylcholine (PC) and in liver and spleen phosphatidylethanolamine (PE). Linolenate was minimally incorporated into PL, but was desaturated and elongated to 20:5n-3, 22:5n-3, and 22:6n-3, with notable differences in the quantity of these n-3 derivatives associated with different tissues and PL. Eicosatrientoate was uniquely incorporated into the cardiolipin (CL) pool of all organs. There was also considerable retroconversion of 20:3n-3 to 18:3n-3 (PC, PE). Dietary eicosatrienoate may therefore affect metabolism in diverse ways-20:3n-3, which is retroconverted to 18:3n-3, may provide substrate for 20:5n-3 and 22:6n-3 syntheses, whereas intact 20:3n-3 may be incorporated into the CL pool. Acyl modifications of CL are known to affect the activity of key innermitochondrial enzymes, such as cytochrome c oxidase.

UR - http://www.scopus.com/inward/record.url?scp=0025144099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025144099&partnerID=8YFLogxK

U2 - 10.1007/BF02538091

DO - 10.1007/BF02538091

M3 - Article

C2 - 1977067

AN - SCOPUS:0025144099

VL - 25

SP - 473

EP - 480

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 8

ER -