Phospholipase D signaling is essential for meiosis

Kristine Rose, Simon A. Rudge, Michael A. Frohman, Andrew J. Morris, Joanne Engebrecht

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SPO14 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. and Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SPO14 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5- bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SPO14 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.

Original languageEnglish (US)
Pages (from-to)12151-12155
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number26
DOIs
StatePublished - Dec 19 1995
Externally publishedYes

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Phospholipase D
Meiosis
Prophase
Phosphatidic Acids
Second Messenger Systems
Enzymes
Phosphatidylinositols
Choline
Phosphatidylcholines
Catalysis
Signal Transduction
Phospholipids
Proteins
Hydrolysis
Yeasts
Alleles
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Phospholipase D signaling is essential for meiosis. / Rose, Kristine; Rudge, Simon A.; Frohman, Michael A.; Morris, Andrew J.; Engebrecht, Joanne.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 26, 19.12.1995, p. 12151-12155.

Research output: Contribution to journalArticle

Rose, Kristine ; Rudge, Simon A. ; Frohman, Michael A. ; Morris, Andrew J. ; Engebrecht, Joanne. / Phospholipase D signaling is essential for meiosis. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 26. pp. 12151-12155.
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