Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SPO14 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. and Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SPO14 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5- bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SPO14 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Dec 19 1995|
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