Phospholipase D function in Saccharomyces cerevisiae

Rima Mendonsa, JoAnne Engebrecht

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Phosphatidylinositol 4,5-bisphosphate-regulated phosphatidylcholine-specific phospholipase D is conserved from yeast to man. The essential role of this enzyme in yeast is to mediate the fusion of Golgi and endosome-derived vesicles to generate the prospore membrane during the developmental program of sporulation, through the production of the fusogenic lipid phosphatidic acid. In addition to recruiting proteins required for fusion, phosphatidic acid is believed to lower the energy barrier to stimulate membrane curvature. During mitotic growth, phospholipase D activity is dispensable unless the major phosphatidylinositol/phosphatidylcholine transfer protein is absent; it also appears to play a nonessential role in the mating signal transduction pathway. The regulation of phospholipase D activity during both sporulation and mitotic growth is still not fully understood and awaits further characterization.

Original languageEnglish (US)
Pages (from-to)970-974
Number of pages5
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1791
Issue number9
DOIs
StatePublished - Sep 2009

Keywords

  • Membrane trafficking
  • Phosphatidic acid
  • Phosphatidylinositol 4,5-bisphosphate
  • Phospholipase D
  • Prospore membrane formation
  • SNARE
  • Sporulation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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