Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects

Zhigang Xie, Min Fang, Marcos P. Rivas, Alexander J. Faulkner, Paul C. Sternweis, Joanne Engebrecht, Vytas A. Bankaitis

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Yeast phosphatidylinositol transfer protein (Sec14p) function is essential for production of Golgi-derived secretory vesicles, and this requirement is bypassed by mutations in at least seven genes. Analyses of such 'bypass Sec14p' mutants suggest that Sec14p acts to maintain an essential Golgi membrane diacylglycerol (DAG) pool that somehow acts to promote Golgi secretory function. SPO14 encodes the sole yeast phosphatidylinositol-4,5-bisphosphate-activated phospholipase D (PLD). PLD function, while essential for meiosis, is dispensable for vegetative growth. Herein, we report specific physiological circumstances under which an unanticipated requirement for PLD activity in yeast vegetative Golgi secretory function is revealed. This PLD involvement is essential in 'bypass Sec14p' mutants where normally Sec14p-dependent Golgi secretory reactions are occurring in a Sec14p-independent manner. PLD catalytic activity is necessary but not sufficient for 'bypass Sec14p', and yeast operating under 'bypass Sec14p' conditions are ethanol-sensitive. These data suggest that PLD supports 'bypass Sec14p' by generating a phosphatidic acid pool that is somehow utilized in supporting yeast Golgi secretory function.

Original languageEnglish (US)
Pages (from-to)12346-12351
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number21
DOIs
StatePublished - Oct 13 1998
Externally publishedYes

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Phospholipid Transfer Proteins
Phospholipase D
Fungal Proteins
Yeasts
Phosphatidic Acids
Diglycerides
Secretory Vesicles
Meiosis
Phosphatidylinositols
Ethanol
Mutation
Membranes
Growth
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. / Xie, Zhigang; Fang, Min; Rivas, Marcos P.; Faulkner, Alexander J.; Sternweis, Paul C.; Engebrecht, Joanne; Bankaitis, Vytas A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 21, 13.10.1998, p. 12346-12351.

Research output: Contribution to journalArticle

Xie, Zhigang ; Fang, Min ; Rivas, Marcos P. ; Faulkner, Alexander J. ; Sternweis, Paul C. ; Engebrecht, Joanne ; Bankaitis, Vytas A. / Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 21. pp. 12346-12351.
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