Phospholipase β4-knockout mouse exhibits retinal phenotype

Huiping Jiang, A. Lyubarsky, N. Vardi, Edward N Pugh Jr, J. Chen, J. Xu, M. I. Simon, Dianqing Wu

Research output: Contribution to journalArticle

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Abstract

Purpose: Determine if PLC-β4 has a retinal function by making/assessing a mouse knockout. Rationale: PLC-β4 is one of the four PLC-β isoforms that have been cloned and can be activated by the Gα subunits of G-proteins of the Gq class, but not by the Gβγ subunits. PLC-β4 shares a closer homology to the NorpA protein (which mediates phototransduction in Drosopnila) than to the isoforms PLC-β1-β3. Previous immunohistochemical studies have shown that PLC-β4 is expressed in cone photoreceptors, and in bipolar and ganglion cells1. Method: A mouse line was generated in which the PLC-β4 genes are disrupted. Retinal rod function was assessed with single-flash a- and b-wave electroretinography. Anatomical analysis of rod density, rod outer segment length and other basic parameters is underway. Results: Extracts of retinal and brain tissues of two animals from each genotype (+/+, +/-, -/-) were examined. Western analysis with a PLC-β4 - specific antibody found no detectable PLC-β4 protein in -/- animals. ERGs of three -/- mice age12-15 weeks showed a-wave maximum amplitudes (amax reduced about 7-8 fold from controls (amax = 460 ± 140 μV), though a-wave sensitivity to light was approximately normal. Both rod and cone b-wave components were severely diminished in amplitude. Preleminary examination of the retinas of the animals whose ERGs were recorded suggests some retinal deterioration has occurred in these animals. Conclusion: The preliminary results demonstrate a requirement for PLC-β4 in normal retinal function. Developmental, anatomical and functional analysis now underway may help to determine the specific role of PLC-β4.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Phospholipases
Knockout Mice
Phenotype
Protein Isoforms
Gq-G11 GTP-Binding Protein alpha Subunits
Light Signal Transduction
Retinal Cone Photoreceptor Cells
Rod Cell Outer Segment
Retinal Rod Photoreceptor Cells
Electroretinography
Photophobia
Vertebrate Photoreceptor Cells
Ganglia
Retina
Proteins
Genotype
Antibodies
Brain
Genes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Jiang, H., Lyubarsky, A., Vardi, N., Pugh Jr, E. N., Chen, J., Xu, J., ... Wu, D. (1996). Phospholipase β4-knockout mouse exhibits retinal phenotype. Investigative Ophthalmology and Visual Science, 37(3).

Phospholipase β4-knockout mouse exhibits retinal phenotype. / Jiang, Huiping; Lyubarsky, A.; Vardi, N.; Pugh Jr, Edward N; Chen, J.; Xu, J.; Simon, M. I.; Wu, Dianqing.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Jiang, H, Lyubarsky, A, Vardi, N, Pugh Jr, EN, Chen, J, Xu, J, Simon, MI & Wu, D 1996, 'Phospholipase β4-knockout mouse exhibits retinal phenotype', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Jiang, Huiping ; Lyubarsky, A. ; Vardi, N. ; Pugh Jr, Edward N ; Chen, J. ; Xu, J. ; Simon, M. I. ; Wu, Dianqing. / Phospholipase β4-knockout mouse exhibits retinal phenotype. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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