Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production

Ebenezer N. Yamoah, Peter G. Gillespie

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To ensure optimal sensitivity for mechanoelectrical transduction, hair cells adapt to prolonged stimuli using active motors. Adaptation motors are thought to employ myosin molecules as their force-producing components. We find that beryllium fluoride, vanadate, and sulfate, phosphate analogs that inhibit the ATPase activity of myosin, inhibit adaptation by abolishing motor force production. Phosphate analogs interact with a 120-kDa bundle protein, most likely myosin Iβ, in a manner that coincides with their effects on adaptation. Features of transduction following inhibition of motor force production suggest that the gating and extent springs of the hair cell orient in parallel at rest and that the negative limit of adaptation arises when force in the stretched extent spring matches the force output of the adaptation motor.

Original languageEnglish (US)
Pages (from-to)523-533
Number of pages11
JournalNeuron
Volume17
Issue number3
DOIs
StatePublished - Sep 1996
Externally publishedYes

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Myosins
Myosin Type I
Phosphates
Vanadates
Proteins
beryllium sulfate
beryllium fluoride

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production. / Yamoah, Ebenezer N.; Gillespie, Peter G.

In: Neuron, Vol. 17, No. 3, 09.1996, p. 523-533.

Research output: Contribution to journalArticle

Yamoah, Ebenezer N. ; Gillespie, Peter G. / Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production. In: Neuron. 1996 ; Vol. 17, No. 3. pp. 523-533.
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