Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse

Rolf Herrmann, Ekaterina S. Lobanova, Timothy Hammond, Christopher Kessler, Marie E Burns, Laura J. Frishman, Vadim Y. Arshavsky

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The rate of synaptic transmission between photoreceptors and bipolar cells has been long known to depend on conditions of ambient illumination. However, the molecular mechanisms that mediate and regulate transmission at this ribbon synapse are poorly understood. We conducted electroretinographic recordings from dark- and light-adapted mice lacking the abundant photoreceptor-specific protein phosducin and found that the ON-bipolar cell responses in these animals have a reduced light sensitivity in the dark-adapted state. Additional desensitization of their responses, normally caused by steady background illumination, was also diminished compared with wild-type animals. This effect was observed in both rod- and cone-driven pathways, with the latter affected to a larger degree. The underlying mechanism is likely to be photoreceptor specific because phosducin is not expressed in other retina neurons and transgenic expression of phosducin in rods of phosducin knock-out mice rescued the rod-specific phenotype. The underlying mechanism functions downstream from the phototransduction cascade, as evident from the sensitivity of phototransduction in phosducin knock-out rods being affected to a much lesser degree than b-wave responses. These data indicate that a major regulatory component responsible for setting the sensitivity of signal transmission between photoreceptors and ON-bipolar cells is confined to photoreceptors and that phosducin participates in the underlying molecular mechanism.

Original languageEnglish (US)
Pages (from-to)3239-3253
Number of pages15
JournalJournal of Neuroscience
Volume30
Issue number9
DOIs
StatePublished - Mar 3 2010

Fingerprint

Synapses
Light Signal Transduction
Lighting
Photophobia
Photoreceptor Cells
Vertebrate Photoreceptor Cells
Wild Animals
Knockout Mice
Synaptic Transmission
Retina
phosducin
Phenotype
Neurons
Light
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Herrmann, R., Lobanova, E. S., Hammond, T., Kessler, C., Burns, M. E., Frishman, L. J., & Arshavsky, V. Y. (2010). Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse. Journal of Neuroscience, 30(9), 3239-3253. https://doi.org/10.1523/JNEUROSCI.4775-09.2010

Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse. / Herrmann, Rolf; Lobanova, Ekaterina S.; Hammond, Timothy; Kessler, Christopher; Burns, Marie E; Frishman, Laura J.; Arshavsky, Vadim Y.

In: Journal of Neuroscience, Vol. 30, No. 9, 03.03.2010, p. 3239-3253.

Research output: Contribution to journalArticle

Herrmann, R, Lobanova, ES, Hammond, T, Kessler, C, Burns, ME, Frishman, LJ & Arshavsky, VY 2010, 'Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse', Journal of Neuroscience, vol. 30, no. 9, pp. 3239-3253. https://doi.org/10.1523/JNEUROSCI.4775-09.2010
Herrmann, Rolf ; Lobanova, Ekaterina S. ; Hammond, Timothy ; Kessler, Christopher ; Burns, Marie E ; Frishman, Laura J. ; Arshavsky, Vadim Y. / Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 9. pp. 3239-3253.
@article{d1334ac1d84c4830b2f4d5e9cbb8f688,
title = "Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse",
abstract = "The rate of synaptic transmission between photoreceptors and bipolar cells has been long known to depend on conditions of ambient illumination. However, the molecular mechanisms that mediate and regulate transmission at this ribbon synapse are poorly understood. We conducted electroretinographic recordings from dark- and light-adapted mice lacking the abundant photoreceptor-specific protein phosducin and found that the ON-bipolar cell responses in these animals have a reduced light sensitivity in the dark-adapted state. Additional desensitization of their responses, normally caused by steady background illumination, was also diminished compared with wild-type animals. This effect was observed in both rod- and cone-driven pathways, with the latter affected to a larger degree. The underlying mechanism is likely to be photoreceptor specific because phosducin is not expressed in other retina neurons and transgenic expression of phosducin in rods of phosducin knock-out mice rescued the rod-specific phenotype. The underlying mechanism functions downstream from the phototransduction cascade, as evident from the sensitivity of phototransduction in phosducin knock-out rods being affected to a much lesser degree than b-wave responses. These data indicate that a major regulatory component responsible for setting the sensitivity of signal transmission between photoreceptors and ON-bipolar cells is confined to photoreceptors and that phosducin participates in the underlying molecular mechanism.",
author = "Rolf Herrmann and Lobanova, {Ekaterina S.} and Timothy Hammond and Christopher Kessler and Burns, {Marie E} and Frishman, {Laura J.} and Arshavsky, {Vadim Y.}",
year = "2010",
month = "3",
day = "3",
doi = "10.1523/JNEUROSCI.4775-09.2010",
language = "English (US)",
volume = "30",
pages = "3239--3253",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "9",

}

TY - JOUR

T1 - Phosducin regulates transmission at the photoreceptor-to-ON-bipolar cell synapse

AU - Herrmann, Rolf

AU - Lobanova, Ekaterina S.

AU - Hammond, Timothy

AU - Kessler, Christopher

AU - Burns, Marie E

AU - Frishman, Laura J.

AU - Arshavsky, Vadim Y.

PY - 2010/3/3

Y1 - 2010/3/3

N2 - The rate of synaptic transmission between photoreceptors and bipolar cells has been long known to depend on conditions of ambient illumination. However, the molecular mechanisms that mediate and regulate transmission at this ribbon synapse are poorly understood. We conducted electroretinographic recordings from dark- and light-adapted mice lacking the abundant photoreceptor-specific protein phosducin and found that the ON-bipolar cell responses in these animals have a reduced light sensitivity in the dark-adapted state. Additional desensitization of their responses, normally caused by steady background illumination, was also diminished compared with wild-type animals. This effect was observed in both rod- and cone-driven pathways, with the latter affected to a larger degree. The underlying mechanism is likely to be photoreceptor specific because phosducin is not expressed in other retina neurons and transgenic expression of phosducin in rods of phosducin knock-out mice rescued the rod-specific phenotype. The underlying mechanism functions downstream from the phototransduction cascade, as evident from the sensitivity of phototransduction in phosducin knock-out rods being affected to a much lesser degree than b-wave responses. These data indicate that a major regulatory component responsible for setting the sensitivity of signal transmission between photoreceptors and ON-bipolar cells is confined to photoreceptors and that phosducin participates in the underlying molecular mechanism.

AB - The rate of synaptic transmission between photoreceptors and bipolar cells has been long known to depend on conditions of ambient illumination. However, the molecular mechanisms that mediate and regulate transmission at this ribbon synapse are poorly understood. We conducted electroretinographic recordings from dark- and light-adapted mice lacking the abundant photoreceptor-specific protein phosducin and found that the ON-bipolar cell responses in these animals have a reduced light sensitivity in the dark-adapted state. Additional desensitization of their responses, normally caused by steady background illumination, was also diminished compared with wild-type animals. This effect was observed in both rod- and cone-driven pathways, with the latter affected to a larger degree. The underlying mechanism is likely to be photoreceptor specific because phosducin is not expressed in other retina neurons and transgenic expression of phosducin in rods of phosducin knock-out mice rescued the rod-specific phenotype. The underlying mechanism functions downstream from the phototransduction cascade, as evident from the sensitivity of phototransduction in phosducin knock-out rods being affected to a much lesser degree than b-wave responses. These data indicate that a major regulatory component responsible for setting the sensitivity of signal transmission between photoreceptors and ON-bipolar cells is confined to photoreceptors and that phosducin participates in the underlying molecular mechanism.

UR - http://www.scopus.com/inward/record.url?scp=77749333416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77749333416&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.4775-09.2010

DO - 10.1523/JNEUROSCI.4775-09.2010

M3 - Article

VL - 30

SP - 3239

EP - 3253

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 9

ER -