Abstract
Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 ± 2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flowcytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include γδ T cells, and CD4-CD8 - and CD8αα+ T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4-CD8- T cells in newborn dogs whereas mature CD4+ and CD8+ T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.
| Original language | English (US) |
|---|---|
| Journal | Veterinary Research |
| Volume | 40 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2009 |
Fingerprint
Keywords
- Dog
- Intestine
- Intraepithelial lymphocyte
ASJC Scopus subject areas
- veterinary(all)
Cite this
Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes. / Luckschander, Nicole; Pfammatter, Nadia S.; Sidler, Daniel; Jakob, Sabine; Burgener, Iwan A.; Moore, Peter F; Zurbriggen, Andreas; Corazza, Nadia; Brunner, Thomas.
In: Veterinary Research, Vol. 40, No. 6, 11.2009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes
AU - Luckschander, Nicole
AU - Pfammatter, Nadia S.
AU - Sidler, Daniel
AU - Jakob, Sabine
AU - Burgener, Iwan A.
AU - Moore, Peter F
AU - Zurbriggen, Andreas
AU - Corazza, Nadia
AU - Brunner, Thomas
PY - 2009/11
Y1 - 2009/11
N2 - Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 ± 2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flowcytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include γδ T cells, and CD4-CD8 - and CD8αα+ T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4-CD8- T cells in newborn dogs whereas mature CD4+ and CD8+ T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.
AB - Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 ± 2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flowcytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include γδ T cells, and CD4-CD8 - and CD8αα+ T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4-CD8- T cells in newborn dogs whereas mature CD4+ and CD8+ T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.
KW - Dog
KW - Intestine
KW - Intraepithelial lymphocyte
UR - http://www.scopus.com/inward/record.url?scp=70349554317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349554317&partnerID=8YFLogxK
U2 - 10.1051/vetres/2009042
DO - 10.1051/vetres/2009042
M3 - Article
C2 - 19631032
AN - SCOPUS:70349554317
VL - 40
JO - Annales de Recherches Veterinaires
JF - Annales de Recherches Veterinaires
SN - 0928-4249
IS - 6
ER -