Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes

Nicole Luckschander, Nadia S. Pfammatter, Daniel Sidler, Sabine Jakob, Iwan A. Burgener, Peter F Moore, Andreas Zurbriggen, Nadia Corazza, Thomas Brunner

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 ± 2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flowcytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include γδ T cells, and CD4-CD8 - and CD8αα+ T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4-CD8- T cells in newborn dogs whereas mature CD4+ and CD8+ T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.

Original languageEnglish (US)
JournalVeterinary Research
Volume40
Issue number6
DOIs
StatePublished - Nov 2009

Keywords

  • Dog
  • Intestine
  • Intraepithelial lymphocyte

ASJC Scopus subject areas

  • veterinary(all)

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