Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis

C. Sugimoto, Patricia A Conrad, L. Mutharia, T. T. Dolan, W. C. Brown, B. M. Goddeeris, T. W. Pearson

Research output: Contribution to journalArticle

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Abstract

Biosynthetically radiolabelled Theileria parva schizonts were purified from bovine lymphoblastoid cells and their proteins were analyzed by two-dimensional gel electrophoresis and autoradiography. The protein spot patterns of schizont proteins from three stocks of T. parva parva indicated that the phenotypic diversity among the stocks was minimal, with the Mariakani and Uganda stocks being identical and the Muguga stock showing only a few differences in minor spots. Comparison of the spot patterns of schizonts of three T. parva subspecies showed that T. p. parva and T. p. bovis differed in only one protein and thus could not be reliably distinguished on the basis of their protein differences. However, T. p. lawrencei showed several protein differences and could be distinguished easily from the other subspecies. Differences in schizont-protein spot patterns were also seen when two different cell lines were infected with the same Theileria stabilate, when one cell line was infected with two different stabilates of the same stock and when uncloned and cloned infected cell lines were used. These results suggest the possibility that selection of phenotypically different parasites could occur in vivo or in vitro.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalParasitology Research
Volume76
Issue number1
DOIs
StatePublished - Jan 1989
Externally publishedYes

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Theileria parva
Schizonts
schizonts
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
Proteins
proteins
cell lines
Cell Line
Theileria
Uganda
autoradiography
Autoradiography
Parasites
parasites
cattle

ASJC Scopus subject areas

  • Parasitology

Cite this

Sugimoto, C., Conrad, P. A., Mutharia, L., Dolan, T. T., Brown, W. C., Goddeeris, B. M., & Pearson, T. W. (1989). Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis. Parasitology Research, 76(1), 1-7. https://doi.org/10.1007/BF00931063

Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis. / Sugimoto, C.; Conrad, Patricia A; Mutharia, L.; Dolan, T. T.; Brown, W. C.; Goddeeris, B. M.; Pearson, T. W.

In: Parasitology Research, Vol. 76, No. 1, 01.1989, p. 1-7.

Research output: Contribution to journalArticle

Sugimoto, C, Conrad, PA, Mutharia, L, Dolan, TT, Brown, WC, Goddeeris, BM & Pearson, TW 1989, 'Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis', Parasitology Research, vol. 76, no. 1, pp. 1-7. https://doi.org/10.1007/BF00931063
Sugimoto, C. ; Conrad, Patricia A ; Mutharia, L. ; Dolan, T. T. ; Brown, W. C. ; Goddeeris, B. M. ; Pearson, T. W. / Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis. In: Parasitology Research. 1989 ; Vol. 76, No. 1. pp. 1-7.
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