Phenotypic Characterization of Lymphocyte Subpopulations in Horses Affected with Chronic Obstructive Pulmonary Disease and in Normal Controls

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The alterations in lymphocyte subsets in chronic obstructive pulmonary disease (COPD) in the horse were investigated by using monoclonal antibodies to identify CD3+, CD4+, CD8+, and surface immunoglobulin positive (sIg+) lymphocytes in peripheral blood, bronchoalveolar lavage fluid (BALF), and pulmonary biopsy frozen tissue sections. COPD-affected horses (n = 5) and normal controls (n = 5) were sampled prestabling and 14 days poststabling, at which time the COPD-affected horses were exhibiting clinical signs of COPD. The peripheral blood absolute CD4+ lymphocyte count was significantly elevated in the COPD-affected horses pre- and poststabling. The CD4 : CD8 ratio in peripheral blood of COPD-affected horses was unaffected by stabling, but the same ratio in the control horses was significantly decreased. These findings support a hypothesis of deficient regulation of a systemic immune response to indoor air in the COPD-affected horses. A large population of leukocytes in pulmonary biopsy immunohistochemical sections from both groups of horses appeared to be CD3+ CD4- CD8-, an uncommon phenotype in both the peripheral blood and BALF.

Original languageEnglish (US)
Pages (from-to)108-116
Number of pages9
JournalVeterinary Pathology
Volume34
Issue number2
StatePublished - Mar 1997

Fingerprint

Lymphocyte Subsets
respiratory tract diseases
Chronic Obstructive Pulmonary Disease
Horses
lymphocytes
horses
blood
Bronchoalveolar Lavage Fluid
biopsy
lungs
Biopsy
Lung
CD4-CD8 Ratio
B-Cell Antigen Receptors
lymphocyte count
Frozen Sections
CD4 Lymphocyte Count
immunoglobulins
leukocytes
monoclonal antibodies

Keywords

  • COPD
  • Flow cytometery
  • Horses
  • Immunohistochemistry
  • Lung
  • Lymphocyte
  • T cell

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{26f76459021e494989029cc5363f6b60,
title = "Phenotypic Characterization of Lymphocyte Subpopulations in Horses Affected with Chronic Obstructive Pulmonary Disease and in Normal Controls",
abstract = "The alterations in lymphocyte subsets in chronic obstructive pulmonary disease (COPD) in the horse were investigated by using monoclonal antibodies to identify CD3+, CD4+, CD8+, and surface immunoglobulin positive (sIg+) lymphocytes in peripheral blood, bronchoalveolar lavage fluid (BALF), and pulmonary biopsy frozen tissue sections. COPD-affected horses (n = 5) and normal controls (n = 5) were sampled prestabling and 14 days poststabling, at which time the COPD-affected horses were exhibiting clinical signs of COPD. The peripheral blood absolute CD4+ lymphocyte count was significantly elevated in the COPD-affected horses pre- and poststabling. The CD4 : CD8 ratio in peripheral blood of COPD-affected horses was unaffected by stabling, but the same ratio in the control horses was significantly decreased. These findings support a hypothesis of deficient regulation of a systemic immune response to indoor air in the COPD-affected horses. A large population of leukocytes in pulmonary biopsy immunohistochemical sections from both groups of horses appeared to be CD3+ CD4- CD8-, an uncommon phenotype in both the peripheral blood and BALF.",
keywords = "COPD, Flow cytometery, Horses, Immunohistochemistry, Lung, Lymphocyte, T cell",
author = "Watson, {Johanna L} and Stott, {Jeffrey L} and Myra Blanchard and Lavoie, {J. P.} and Wilson, {William D} and Gershwin, {Laurel J} and Wilson, {Dennis W}",
year = "1997",
month = "3",
language = "English (US)",
volume = "34",
pages = "108--116",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "2",

}

TY - JOUR

T1 - Phenotypic Characterization of Lymphocyte Subpopulations in Horses Affected with Chronic Obstructive Pulmonary Disease and in Normal Controls

AU - Watson, Johanna L

AU - Stott, Jeffrey L

AU - Blanchard, Myra

AU - Lavoie, J. P.

AU - Wilson, William D

AU - Gershwin, Laurel J

AU - Wilson, Dennis W

PY - 1997/3

Y1 - 1997/3

N2 - The alterations in lymphocyte subsets in chronic obstructive pulmonary disease (COPD) in the horse were investigated by using monoclonal antibodies to identify CD3+, CD4+, CD8+, and surface immunoglobulin positive (sIg+) lymphocytes in peripheral blood, bronchoalveolar lavage fluid (BALF), and pulmonary biopsy frozen tissue sections. COPD-affected horses (n = 5) and normal controls (n = 5) were sampled prestabling and 14 days poststabling, at which time the COPD-affected horses were exhibiting clinical signs of COPD. The peripheral blood absolute CD4+ lymphocyte count was significantly elevated in the COPD-affected horses pre- and poststabling. The CD4 : CD8 ratio in peripheral blood of COPD-affected horses was unaffected by stabling, but the same ratio in the control horses was significantly decreased. These findings support a hypothesis of deficient regulation of a systemic immune response to indoor air in the COPD-affected horses. A large population of leukocytes in pulmonary biopsy immunohistochemical sections from both groups of horses appeared to be CD3+ CD4- CD8-, an uncommon phenotype in both the peripheral blood and BALF.

AB - The alterations in lymphocyte subsets in chronic obstructive pulmonary disease (COPD) in the horse were investigated by using monoclonal antibodies to identify CD3+, CD4+, CD8+, and surface immunoglobulin positive (sIg+) lymphocytes in peripheral blood, bronchoalveolar lavage fluid (BALF), and pulmonary biopsy frozen tissue sections. COPD-affected horses (n = 5) and normal controls (n = 5) were sampled prestabling and 14 days poststabling, at which time the COPD-affected horses were exhibiting clinical signs of COPD. The peripheral blood absolute CD4+ lymphocyte count was significantly elevated in the COPD-affected horses pre- and poststabling. The CD4 : CD8 ratio in peripheral blood of COPD-affected horses was unaffected by stabling, but the same ratio in the control horses was significantly decreased. These findings support a hypothesis of deficient regulation of a systemic immune response to indoor air in the COPD-affected horses. A large population of leukocytes in pulmonary biopsy immunohistochemical sections from both groups of horses appeared to be CD3+ CD4- CD8-, an uncommon phenotype in both the peripheral blood and BALF.

KW - COPD

KW - Flow cytometery

KW - Horses

KW - Immunohistochemistry

KW - Lung

KW - Lymphocyte

KW - T cell

UR - http://www.scopus.com/inward/record.url?scp=0031092217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031092217&partnerID=8YFLogxK

M3 - Article

C2 - 9066077

AN - SCOPUS:0031092217

VL - 34

SP - 108

EP - 116

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 2

ER -