Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean islands

Lucy Chou, Matt J. Griffin, Trellor Fraites, Cynthia Ware, Hugh Ferguson, Natalie Keirstead, John Brake, Judy Wiles, John P. Hawke, Michael T. Kearney, Rodman G. Getchell, Patricia Gaunt, Esteban Soto Martinez

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Streptococcus iniae, the etiological agent of streptococcosis in fish, is an important pathogen of cultured and wild fish worldwide. During the last decade outbreaks of streptococcosis have occurred in a wide range of cultured and wild fish in the Americas and Caribbean islands. To gain a better understanding of the epizootiology of S. iniae in the western hemisphere, over 30 S. iniae isolates recovered from different fish species and geographic locations were characterized phenotypically and genetically. Species identities were determined biochemically and confirmed by amplification and sequencing of the 16S rRNA gene. Repetitive-element palindromic PCR fingerprinting as well as biochemical and antimicrobial susceptibility profiles suggest that a single strain of S. iniae was responsible for two different disease outbreaks among reef fishes in the Caribbean, one in 1999 and another in 2008. Interestingly, a majority of the isolates recovered from cultured fish in the Americas were genetically distinct from the Caribbean isolates and exhibited a trend toward higher minimal inhibitory concentration with respect to several antibiotics as well as greater genetic variability. The biological significance of this genetic variability is unclear, but it could have implications for future vaccine development and treatment.

Original languageEnglish (US)
Pages (from-to)263-271
Number of pages9
JournalJournal of Aquatic Animal Health
Volume26
Issue number4
DOIs
StatePublished - Oct 31 2014
Externally publishedYes

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Streptococcus iniae
West Indies
Central America
wild fish
North America
Fishes
fish
genetic variation
Disease Outbreaks
etiological agents
vaccine development
epidemiology
Geographic Locations
reefs
anti-infective agents
antibiotics
vaccine
ribosomal RNA
rRNA Genes
pathogens

ASJC Scopus subject areas

  • Aquatic Science
  • Medicine(all)

Cite this

Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean islands. / Chou, Lucy; Griffin, Matt J.; Fraites, Trellor; Ware, Cynthia; Ferguson, Hugh; Keirstead, Natalie; Brake, John; Wiles, Judy; Hawke, John P.; Kearney, Michael T.; Getchell, Rodman G.; Gaunt, Patricia; Soto Martinez, Esteban.

In: Journal of Aquatic Animal Health, Vol. 26, No. 4, 31.10.2014, p. 263-271.

Research output: Contribution to journalArticle

Chou, L, Griffin, MJ, Fraites, T, Ware, C, Ferguson, H, Keirstead, N, Brake, J, Wiles, J, Hawke, JP, Kearney, MT, Getchell, RG, Gaunt, P & Soto Martinez, E 2014, 'Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean islands', Journal of Aquatic Animal Health, vol. 26, no. 4, pp. 263-271. https://doi.org/10.1080/08997659.2014.945048
Chou, Lucy ; Griffin, Matt J. ; Fraites, Trellor ; Ware, Cynthia ; Ferguson, Hugh ; Keirstead, Natalie ; Brake, John ; Wiles, Judy ; Hawke, John P. ; Kearney, Michael T. ; Getchell, Rodman G. ; Gaunt, Patricia ; Soto Martinez, Esteban. / Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean islands. In: Journal of Aquatic Animal Health. 2014 ; Vol. 26, No. 4. pp. 263-271.
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