Phenotypic and genotypic characterization of klebsiella pneumoniae isolates recovered from nonhuman primates

Esteban Soto Martinez, Virginia Lamon, Matt Griffin, Natalie Keirstead, Amy Beierschmitt, Roberta Palmour

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Klebsiella pneumoniae is a zoonotic, Gram-negative member of the family Enterobacteriaceae and is the causative agent of nosocomial septicemic, pneumonic, and urinary tract infections. Recently, pathogenic strains of K. pneumoniae sharing a hypermucoviscosity (HMV) phenotype have been attributed to multisystemic abscessation in both human and nonhuman primates. Although K. pneumoniae is a well-recognized zoonotic agent, there is a lack of general information including adequate diagnostic methods or treatments for nonhuman primates. In an effort to increase the body of knowledge of this enigmatic pathogen, K. pneumoniae isolates from African green monkeys (Chlorocebus aethiops sabaeus) on the island of St. Kitts, West Indies were genotypically and phenotypically characterized. Genetic fingerprints generated by PCRmediated genomic fingerprinting, phenotypic characterization, and antimicrobial susceptibility all identified a high degree of similarity between the HMV and non-HMV K. pneumoniae isolates. The results obtained from this work will help establish a baseline for the development of efficacious diagnostic methods and treatment strategies for both human and nonhuman primates.

Original languageEnglish (US)
Pages (from-to)603-611
Number of pages9
JournalJournal of Wildlife Diseases
Volume48
Issue number3
StatePublished - Jul 2012
Externally publishedYes

Fingerprint

pneumonia
Klebsiella pneumoniae
primate
Primates
Cercopithecus aethiops
diagnostic techniques
urinary tract diseases
Enterobacteriaceae
phenotype
genomics
pathogen
anti-infective agents
lungs
pathogens

Keywords

  • Antimicrobial susceptibility
  • Green vervet monkey
  • Hypermucoviscosity
  • Klebsiella pneumoniae

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Phenotypic and genotypic characterization of klebsiella pneumoniae isolates recovered from nonhuman primates. / Soto Martinez, Esteban; Lamon, Virginia; Griffin, Matt; Keirstead, Natalie; Beierschmitt, Amy; Palmour, Roberta.

In: Journal of Wildlife Diseases, Vol. 48, No. 3, 07.2012, p. 603-611.

Research output: Contribution to journalArticle

Soto Martinez, E, Lamon, V, Griffin, M, Keirstead, N, Beierschmitt, A & Palmour, R 2012, 'Phenotypic and genotypic characterization of klebsiella pneumoniae isolates recovered from nonhuman primates', Journal of Wildlife Diseases, vol. 48, no. 3, pp. 603-611.
Soto Martinez, Esteban ; Lamon, Virginia ; Griffin, Matt ; Keirstead, Natalie ; Beierschmitt, Amy ; Palmour, Roberta. / Phenotypic and genotypic characterization of klebsiella pneumoniae isolates recovered from nonhuman primates. In: Journal of Wildlife Diseases. 2012 ; Vol. 48, No. 3. pp. 603-611.
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