Phenotypic analysis of skin infiltrates in comparison with peripheral blood lymphocytes, spleen cells and thymocytes in early avian scleroderma

Matthias S. Gruschwitz, Susanne Moormann, Guido Krömer, Roswitha Sgonc, Herrmann Dietrich, Günther Boeck, M. Eric Gershwin, Richard Boyd, Georg Wick

Research output: Contribution to journalArticle

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Abstract

University of California at Davis line 200 (UCD-200) chickens develop a hereditary connective tissue disease characterized by severe lymphocytic infiltration, vascular occlusion and fibrosis of skin and internal organs. To identify cellular immunological abnormalities in the acute inflammatory disease stage of this animal model for progressive systemic sclerosis (PSS) we investigated the phenotypic characteristics and function of peripheral blood lymphocytes (PBL), spleen cells and thymocytes in comparison with skin infiltrating cells. Immunofluorescence and immunohistochemical analysis using monoclonal antibodies revealed the overwhelming majority of skin infiltrating mononuclear cells in the deeper dermis and subcutaneous tissue to be T cell receptor α β (TcR2)+/CD3+/CD4+/class II+ cells, a small portion (5-10%) of which were interleukin 2 (IL-2) receptor positive. In contrast, the inflammatory infiltrate in perivascular areas of the papillary dermis was constituted of mainly TcR γ δ (TcR1)+/class II- lymphocytes. Only few B cells ( T B cell ratio > 5) were detected. These diseased chickens showed significantly reduced percentages and numbers of circulating peripheral T cells exhibiting TcR1, TcR2, CD3, CD4 or IL-2-receptor, probably owing to an increased influx into lymphoid organs and affected tissues. In contrast to healthy chickens, the thymi of UCD-200 animals revealed fewer cells expressing TcR1, TcR2 and class II antigen, suggesting an altered intrathymic maturation of the T cell lineage. Functional in vitro studies showed a significantly decreased T cell mitogen-induced proliferation rate associated with a decreased capacity to produce IL-2 and to express IL-2 receptors. In contrast to the deficient in vitro IL-2 production the sera of UCD-200 chickens contained significant levels of IL-2 bioactivity. The alteration of T lymphocyte physiology in UCD-200 chickens adds, at least in part, to the parallels between this animal model and its human counterpart. These data confirm our hypothesis that the PSS-like disease of UCD-200 chickens includes a numeric and/or functional alteration of peripheral T cell subsets, especially of TcR1 positive cells, in contrast to the pronounced accumulation in the afflicted tissues.

Original languageEnglish (US)
Pages (from-to)577-593
Number of pages17
JournalJournal of Autoimmunity
Volume4
Issue number4
DOIs
StatePublished - 1991

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Thymocytes
Chickens
Spleen
Lymphocytes
Skin
Interleukin-2 Receptors
Interleukin-2
T-Lymphocytes
Diffuse Scleroderma
Dermis
B-Lymphocytes
Animal Models
Connective Tissue Diseases
Histocompatibility Antigens Class II
Subcutaneous Tissue
T-Lymphocyte Subsets
Acute Disease
Cell Lineage
T-Cell Antigen Receptor
Mitogens

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Phenotypic analysis of skin infiltrates in comparison with peripheral blood lymphocytes, spleen cells and thymocytes in early avian scleroderma. / Gruschwitz, Matthias S.; Moormann, Susanne; Krömer, Guido; Sgonc, Roswitha; Dietrich, Herrmann; Boeck, Günther; Gershwin, M. Eric; Boyd, Richard; Wick, Georg.

In: Journal of Autoimmunity, Vol. 4, No. 4, 1991, p. 577-593.

Research output: Contribution to journalArticle

Gruschwitz, Matthias S. ; Moormann, Susanne ; Krömer, Guido ; Sgonc, Roswitha ; Dietrich, Herrmann ; Boeck, Günther ; Gershwin, M. Eric ; Boyd, Richard ; Wick, Georg. / Phenotypic analysis of skin infiltrates in comparison with peripheral blood lymphocytes, spleen cells and thymocytes in early avian scleroderma. In: Journal of Autoimmunity. 1991 ; Vol. 4, No. 4. pp. 577-593.
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