Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa

Ming Wen Li, John D. Biggers, Mehmet Toner, Stephen M Griffey, Kevin C K Lloyd

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Combination of evaporative drying and frozen storage at -80°C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/NJ mice were evaporatively dried and stored for 1 mo at -80°C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After weaning, 1 male and 1 female mouse from each litter were randomly selected at 8 wk of age for natural mating to produce live offspring. Results showed that spermatozoa from both inbred strains that had been evaporatively dried and subsequently stored at -80°C could be used successfully to derive live, healthy, and reproductively sound offspring by ICSI. No significant differences were found in embryo transfer rate (number of pups born/number of embryos transferred), litter size, weaning rate, body weight, number of pathologic lesions, and amount of contamination by pathogens of mice produced by ICSI using evaporatively dried spermatozoa compared with mice produced by natural mating or by ICSI using fresh (that is, nonpreserved) spermatozoa. Progeny produced by mating mice generated from ICSI using evaporatively dried spermatozoa were normal. Therefore, spermatozoa from inbred mouse strains C57BL/6J and FVB/NJ can be preserved successfully after evaporative drying and frozen storage at -80°C.

Original languageEnglish (US)
Pages (from-to)469-475
Number of pages7
JournalComparative Medicine
Volume57
Issue number5
StatePublished - Oct 2007

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Spermatozoa
Intracytoplasmic Sperm Injections
intracytoplasmic sperm injection
Drying
spermatozoa
mice
Pathogens
Contamination
Acoustic waves
natural mating
Weaning
frozen storage
weaning
drying
Litter Size
Inbred Strains Mice
Embryo Transfer
Inbred C57BL Mouse
embryo transfer
litters (young animals)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. / Li, Ming Wen; Biggers, John D.; Toner, Mehmet; Griffey, Stephen M; Lloyd, Kevin C K.

In: Comparative Medicine, Vol. 57, No. 5, 10.2007, p. 469-475.

Research output: Contribution to journalArticle

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