Abstract
Combination of evaporative drying and frozen storage at -80°C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/NJ mice were evaporatively dried and stored for 1 mo at -80°C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After weaning, 1 male and 1 female mouse from each litter were randomly selected at 8 wk of age for natural mating to produce live offspring. Results showed that spermatozoa from both inbred strains that had been evaporatively dried and subsequently stored at -80°C could be used successfully to derive live, healthy, and reproductively sound offspring by ICSI. No significant differences were found in embryo transfer rate (number of pups born/number of embryos transferred), litter size, weaning rate, body weight, number of pathologic lesions, and amount of contamination by pathogens of mice produced by ICSI using evaporatively dried spermatozoa compared with mice produced by natural mating or by ICSI using fresh (that is, nonpreserved) spermatozoa. Progeny produced by mating mice generated from ICSI using evaporatively dried spermatozoa were normal. Therefore, spermatozoa from inbred mouse strains C57BL/6J and FVB/NJ can be preserved successfully after evaporative drying and frozen storage at -80°C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 469-475 |
| Number of pages | 7 |
| Journal | Comparative Medicine |
| Volume | 57 |
| Issue number | 5 |
| State | Published - Oct 2007 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. / Li, Ming Wen; Biggers, John D.; Toner, Mehmet; Griffey, Stephen M; Lloyd, Kevin C K.
In: Comparative Medicine, Vol. 57, No. 5, 10.2007, p. 469-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa
AU - Li, Ming Wen
AU - Biggers, John D.
AU - Toner, Mehmet
AU - Griffey, Stephen M
AU - Lloyd, Kevin C K
PY - 2007/10
Y1 - 2007/10
N2 - Combination of evaporative drying and frozen storage at -80°C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/NJ mice were evaporatively dried and stored for 1 mo at -80°C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After weaning, 1 male and 1 female mouse from each litter were randomly selected at 8 wk of age for natural mating to produce live offspring. Results showed that spermatozoa from both inbred strains that had been evaporatively dried and subsequently stored at -80°C could be used successfully to derive live, healthy, and reproductively sound offspring by ICSI. No significant differences were found in embryo transfer rate (number of pups born/number of embryos transferred), litter size, weaning rate, body weight, number of pathologic lesions, and amount of contamination by pathogens of mice produced by ICSI using evaporatively dried spermatozoa compared with mice produced by natural mating or by ICSI using fresh (that is, nonpreserved) spermatozoa. Progeny produced by mating mice generated from ICSI using evaporatively dried spermatozoa were normal. Therefore, spermatozoa from inbred mouse strains C57BL/6J and FVB/NJ can be preserved successfully after evaporative drying and frozen storage at -80°C.
AB - Combination of evaporative drying and frozen storage at -80°C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/NJ mice were evaporatively dried and stored for 1 mo at -80°C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After weaning, 1 male and 1 female mouse from each litter were randomly selected at 8 wk of age for natural mating to produce live offspring. Results showed that spermatozoa from both inbred strains that had been evaporatively dried and subsequently stored at -80°C could be used successfully to derive live, healthy, and reproductively sound offspring by ICSI. No significant differences were found in embryo transfer rate (number of pups born/number of embryos transferred), litter size, weaning rate, body weight, number of pathologic lesions, and amount of contamination by pathogens of mice produced by ICSI using evaporatively dried spermatozoa compared with mice produced by natural mating or by ICSI using fresh (that is, nonpreserved) spermatozoa. Progeny produced by mating mice generated from ICSI using evaporatively dried spermatozoa were normal. Therefore, spermatozoa from inbred mouse strains C57BL/6J and FVB/NJ can be preserved successfully after evaporative drying and frozen storage at -80°C.
UR - http://www.scopus.com/inward/record.url?scp=38449102854&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38449102854&partnerID=8YFLogxK
M3 - Article
C2 - 17974129
AN - SCOPUS:38449102854
VL - 57
SP - 469
EP - 475
JO - Comparative Medicine
JF - Comparative Medicine
SN - 1532-0820
IS - 5
ER -