Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage

K. L. Carraway, Y. Liu, D. Puett, K. L. Carraway, C. A C Carraway

Research output: Contribution to journalArticle

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Abstract

Microvilli isolated from 13762 mammary ascites tumor cells contain a major calcium-sensitive protein (AMV-p35) that can be isolated with microvillar microfilament cores prepared by Triton X-100 extraction in the presence but not abscence of calcium. AMV-p35 can be readily purified from ethylene glycol bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid extracts of the microfilament cores by chromatography on an anion exchange column, to which it does not bind. Immunoblot analysis indicates that AMV-p35 is related to calpactin I, the pp60(src) tyrosine kinase substrate. In the presence of calcium, AMV-p35 binds approximately 4 mol of chlorpromazine per mole of protein in a binding process showing apparent positive cooperativity, similar to calmodulin; however, in contrast to calmodulin, AMV-p35 also binds phenothiazine in the abscence of calcium.

Original languageEnglish (US)
Pages (from-to)46-50
Number of pages5
JournalFASEB Journal
Volume1
Issue number1
StatePublished - 1987
Externally publishedYes

Fingerprint

phenothiazine
Annexins
src-Family Kinases
tyrosine
phosphotransferases (kinases)
calcium
Calcium
calmodulin
microfilaments
Calmodulin
Actin Cytoskeleton
chlorpromazine
Annexin A2
protein binding
ethylene glycol
ascites
anion exchange
microvilli
Ethylene Glycol
breasts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Carraway, K. L., Liu, Y., Puett, D., Carraway, K. L., & Carraway, C. A. C. (1987). Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage. FASEB Journal, 1(1), 46-50.

Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage. / Carraway, K. L.; Liu, Y.; Puett, D.; Carraway, K. L.; Carraway, C. A C.

In: FASEB Journal, Vol. 1, No. 1, 1987, p. 46-50.

Research output: Contribution to journalArticle

Carraway, KL, Liu, Y, Puett, D, Carraway, KL & Carraway, CAC 1987, 'Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage', FASEB Journal, vol. 1, no. 1, pp. 46-50.
Carraway, K. L. ; Liu, Y. ; Puett, D. ; Carraway, K. L. ; Carraway, C. A C. / Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage. In: FASEB Journal. 1987 ; Vol. 1, No. 1. pp. 46-50.
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