TY - JOUR
T1 - Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons
AU - ffrench-Mullen, J. M H
AU - Rogawski, Michael A
AU - Barker, J. L.
PY - 1988/6/7
Y1 - 1988/6/7
N2 - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.
AB - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.
KW - A-current
KW - Delayed rectifier current
KW - Phencyclidine
KW - Potassium channel
KW - Tetraethylammonium
KW - Whole cell patch clamp
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U2 - 10.1016/0304-3940(88)90232-7
DO - 10.1016/0304-3940(88)90232-7
M3 - Article
C2 - 3386879
AN - SCOPUS:0023895071
VL - 88
SP - 325
EP - 330
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -