Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

J. M H ffrench-Mullen, Michael A Rogawski, J. L. Barker

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.

Original languageEnglish (US)
Pages (from-to)325-330
Number of pages6
JournalNeuroscience Letters
Volume88
Issue number3
DOIs
StatePublished - Jun 7 1988
Externally publishedYes

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Keywords

  • A-current
  • Delayed rectifier current
  • Phencyclidine
  • Potassium channel
  • Tetraethylammonium
  • Whole cell patch clamp

ASJC Scopus subject areas

  • Neuroscience(all)

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