Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

J. M H ffrench-Mullen; Michael A Rogawski; J. L. Barker

doi:10.1016/0304-3940(88)90232-7

Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

J. M H ffrench-Mullen, Michael A Rogawski, J. L. Barker

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We studied the effects of phencyclidine (PCP) on voltage-dependent K⁺ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na⁺ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, I_A, and (ii) a slowly activating, minimally inactivating ('sustained'), component, I_K. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of I_K with minimal effect on I_A; however, at higher concentrations both currents were suppressed. The IC₅₀'s for blockade of I_K and I_A were 36 and 310 μM, respectively.

Original language	English (US)
Pages (from-to)	325-330
Number of pages	6
Journal	Neuroscience Letters
Volume	88
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(88)90232-7
State	Published - Jun 7 1988
Externally published	Yes

Keywords

A-current
Delayed rectifier current
Phencyclidine
Potassium channel
Tetraethylammonium
Whole cell patch clamp

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(88)90232-7

Cite this

@article{842f3f3461694e9ca84c07d7b0cafe02,

title = "Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons",

abstract = "We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.",

keywords = "A-current, Delayed rectifier current, Phencyclidine, Potassium channel, Tetraethylammonium, Whole cell patch clamp",

author = "ffrench-Mullen, {J. M H} and Rogawski, {Michael A} and Barker, {J. L.}",

year = "1988",

month = jun,

day = "7",

doi = "10.1016/0304-3940(88)90232-7",

language = "English (US)",

volume = "88",

pages = "325--330",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

AU - ffrench-Mullen, J. M H

AU - Rogawski, Michael A

AU - Barker, J. L.

PY - 1988/6/7

Y1 - 1988/6/7

N2 - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.

AB - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.

KW - A-current

KW - Delayed rectifier current

KW - Phencyclidine

KW - Potassium channel

KW - Tetraethylammonium

KW - Whole cell patch clamp

UR - http://www.scopus.com/inward/record.url?scp=0023895071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023895071&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(88)90232-7

DO - 10.1016/0304-3940(88)90232-7

M3 - Article

C2 - 3386879

AN - SCOPUS:0023895071

VL - 88

SP - 325

EP - 330

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -

Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this