Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

J. M H ffrench-Mullen; Michael A Rogawski; J. L. Barker

doi:10.1016/0304-3940(88)90232-7

Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

J. M H ffrench-Mullen, Michael A Rogawski, J. L. Barker

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We studied the effects of phencyclidine (PCP) on voltage-dependent K⁺ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na⁺ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, I_A, and (ii) a slowly activating, minimally inactivating ('sustained'), component, I_K. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of I_K with minimal effect on I_A; however, at higher concentrations both currents were suppressed. The IC₅₀'s for blockade of I_K and I_A were 36 and 310 μM, respectively.

Original language	English (US)
Pages (from-to)	325-330
Number of pages	6
Journal	Neuroscience Letters
Volume	88
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(88)90232-7
State	Published - Jun 7 1988
Externally published	Yes

Keywords

A-current
Delayed rectifier current
Phencyclidine
Potassium channel
Tetraethylammonium
Whole cell patch clamp

ASJC Scopus subject areas

Neuroscience(all)

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title = "Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons",

abstract = "We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.",

keywords = "A-current, Delayed rectifier current, Phencyclidine, Potassium channel, Tetraethylammonium, Whole cell patch clamp",

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AU - ffrench-Mullen, J. M H

AU - Rogawski, Michael A

AU - Barker, J. L.

PY - 1988/6/7

Y1 - 1988/6/7

N2 - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.

AB - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.

KW - A-current

KW - Delayed rectifier current

KW - Phencyclidine

KW - Potassium channel

KW - Tetraethylammonium

KW - Whole cell patch clamp

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Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

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