Abstract
We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 325-330 |
| Number of pages | 6 |
| Journal | Neuroscience Letters |
| Volume | 88 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 7 1988 |
| Externally published | Yes |
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Keywords
- A-current
- Delayed rectifier current
- Phencyclidine
- Potassium channel
- Tetraethylammonium
- Whole cell patch clamp
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons. / ffrench-Mullen, J. M H; Rogawski, Michael A; Barker, J. L.
In: Neuroscience Letters, Vol. 88, No. 3, 07.06.1988, p. 325-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phencyclidine at low concentrations selectively blocks the sustained but not the transient voltage-dependent potassium current in cultured hippocampal neurons
AU - ffrench-Mullen, J. M H
AU - Rogawski, Michael A
AU - Barker, J. L.
PY - 1988/6/7
Y1 - 1988/6/7
N2 - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.
AB - We studied the effects of phencyclidine (PCP) on voltage-dependent K+ currents in cultured embryonic rat hippocampal neurons. Whole cell voltage-clamp recordings were made in the presence of tetrodotoxin to block Na+ current. Depolarizing voltage steps activated two outward current components: (i) a rapidly activating and inactivating ('transient') component, IA, and (ii) a slowly activating, minimally inactivating ('sustained'), component, IK. At low concentrations (< 50-100 μM), PCP produced a selective, reversible blockade of IK with minimal effect on IA; however, at higher concentrations both currents were suppressed. The IC50's for blockade of IK and IA were 36 and 310 μM, respectively.
KW - A-current
KW - Delayed rectifier current
KW - Phencyclidine
KW - Potassium channel
KW - Tetraethylammonium
KW - Whole cell patch clamp
UR - http://www.scopus.com/inward/record.url?scp=0023895071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023895071&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(88)90232-7
DO - 10.1016/0304-3940(88)90232-7
M3 - Article
C2 - 3386879
AN - SCOPUS:0023895071
VL - 88
SP - 325
EP - 330
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -