Pharmacological modulation of the antigen-induced expression of the low-affinity IgE receptor (FcεRII/CD23) on rat alveolar macrophages

J. M. Mencia-Huerta, B. Dugas, E. Boichot, C. Petit-Frere, N. V. Paul-Eugene Lagente, M. Capron, F. T. Liu, Fu-Tong Liu

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Abstract

Brown-Norway (BN) rats were sensitized by 3 aerosol exposures to ovalbumin (OA; 10 mg/ml) at days 1, 3 and 14. At day 21, the rats were challenged with the antigen or vehicle by aerosol. Alveolar macrophages (AM) were obtained by bronchoalveolar lavage and the expression of FcεRlI/CD23 was assessed by flow cytometry after staining with the BB10 monoclonal antibody. A maximum of 74% of the AM from sensitized and challenged BN rats expressed FCεRII/CD23 24 h after OA exposure, compared to 12% of the cells from rats exposed to vehicle. Sprague-Dawley rats were passively sensitized by intravenous injection of 0.1 or 0.05 ml/kg mouse ascitic fluid containing dinitrophenyl (DNP)-specific monoclonal IgE (2682-1) and after 24 h exposed to an aerosol of 5 mg/ml of DNP-bovine serum albumin for 30 min. In this case also, antigen exposure induced the expression of FcεRII/CD23 on 75% AM, compared to 17% AM from saline-challenged rats. Such an induction of FcεRII/CD23 on AM was, however, not observed when the animals were challenged with either histamine, serotonin or acetylcholine by aerosol. The antigen-induced expression of FcεRII/CD23 on AM was inhibited upon treatment of the rats with ketotifen or beclomethasone. In addition, oral or aerosol administration of respectively BN 50730 or BN 52021 (two structurally unrelated platelet-activating factor antagonists), inhibited the antigen-induced FcεRII/CD23 expression on AM, indicating the participation of this lipid mediator in this process.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalInternational Archives of Allergy and Applied Immunology
Volume94
Issue number1-4
StatePublished - 1991

Fingerprint

IgE Receptors
Alveolar Macrophages
Aerosols
Pharmacology
Antigens
Norway
Ketotifen
Beclomethasone
Ascitic Fluid
Platelet Activating Factor
Ovalbumin
Bronchoalveolar Lavage
Intravenous Injections
Immunoglobulin E
Histamine
Acetylcholine
Sprague Dawley Rats
Serotonin
Flow Cytometry
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Mencia-Huerta, J. M., Dugas, B., Boichot, E., Petit-Frere, C., Paul-Eugene Lagente, N. V., Capron, M., ... Liu, F-T. (1991). Pharmacological modulation of the antigen-induced expression of the low-affinity IgE receptor (FcεRII/CD23) on rat alveolar macrophages. International Archives of Allergy and Applied Immunology, 94(1-4), 295-298.

Pharmacological modulation of the antigen-induced expression of the low-affinity IgE receptor (FcεRII/CD23) on rat alveolar macrophages. / Mencia-Huerta, J. M.; Dugas, B.; Boichot, E.; Petit-Frere, C.; Paul-Eugene Lagente, N. V.; Capron, M.; Liu, F. T.; Liu, Fu-Tong.

In: International Archives of Allergy and Applied Immunology, Vol. 94, No. 1-4, 1991, p. 295-298.

Research output: Contribution to journalArticle

Mencia-Huerta, JM, Dugas, B, Boichot, E, Petit-Frere, C, Paul-Eugene Lagente, NV, Capron, M, Liu, FT & Liu, F-T 1991, 'Pharmacological modulation of the antigen-induced expression of the low-affinity IgE receptor (FcεRII/CD23) on rat alveolar macrophages', International Archives of Allergy and Applied Immunology, vol. 94, no. 1-4, pp. 295-298.
Mencia-Huerta, J. M. ; Dugas, B. ; Boichot, E. ; Petit-Frere, C. ; Paul-Eugene Lagente, N. V. ; Capron, M. ; Liu, F. T. ; Liu, Fu-Tong. / Pharmacological modulation of the antigen-induced expression of the low-affinity IgE receptor (FcεRII/CD23) on rat alveolar macrophages. In: International Archives of Allergy and Applied Immunology. 1991 ; Vol. 94, No. 1-4. pp. 295-298.
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abstract = "Brown-Norway (BN) rats were sensitized by 3 aerosol exposures to ovalbumin (OA; 10 mg/ml) at days 1, 3 and 14. At day 21, the rats were challenged with the antigen or vehicle by aerosol. Alveolar macrophages (AM) were obtained by bronchoalveolar lavage and the expression of FcεRlI/CD23 was assessed by flow cytometry after staining with the BB10 monoclonal antibody. A maximum of 74{\%} of the AM from sensitized and challenged BN rats expressed FCεRII/CD23 24 h after OA exposure, compared to 12{\%} of the cells from rats exposed to vehicle. Sprague-Dawley rats were passively sensitized by intravenous injection of 0.1 or 0.05 ml/kg mouse ascitic fluid containing dinitrophenyl (DNP)-specific monoclonal IgE (2682-1) and after 24 h exposed to an aerosol of 5 mg/ml of DNP-bovine serum albumin for 30 min. In this case also, antigen exposure induced the expression of FcεRII/CD23 on 75{\%} AM, compared to 17{\%} AM from saline-challenged rats. Such an induction of FcεRII/CD23 on AM was, however, not observed when the animals were challenged with either histamine, serotonin or acetylcholine by aerosol. The antigen-induced expression of FcεRII/CD23 on AM was inhibited upon treatment of the rats with ketotifen or beclomethasone. In addition, oral or aerosol administration of respectively BN 50730 or BN 52021 (two structurally unrelated platelet-activating factor antagonists), inhibited the antigen-induced FcεRII/CD23 expression on AM, indicating the participation of this lipid mediator in this process.",
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AU - Mencia-Huerta, J. M.

AU - Dugas, B.

AU - Boichot, E.

AU - Petit-Frere, C.

AU - Paul-Eugene Lagente, N. V.

AU - Capron, M.

AU - Liu, F. T.

AU - Liu, Fu-Tong

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N2 - Brown-Norway (BN) rats were sensitized by 3 aerosol exposures to ovalbumin (OA; 10 mg/ml) at days 1, 3 and 14. At day 21, the rats were challenged with the antigen or vehicle by aerosol. Alveolar macrophages (AM) were obtained by bronchoalveolar lavage and the expression of FcεRlI/CD23 was assessed by flow cytometry after staining with the BB10 monoclonal antibody. A maximum of 74% of the AM from sensitized and challenged BN rats expressed FCεRII/CD23 24 h after OA exposure, compared to 12% of the cells from rats exposed to vehicle. Sprague-Dawley rats were passively sensitized by intravenous injection of 0.1 or 0.05 ml/kg mouse ascitic fluid containing dinitrophenyl (DNP)-specific monoclonal IgE (2682-1) and after 24 h exposed to an aerosol of 5 mg/ml of DNP-bovine serum albumin for 30 min. In this case also, antigen exposure induced the expression of FcεRII/CD23 on 75% AM, compared to 17% AM from saline-challenged rats. Such an induction of FcεRII/CD23 on AM was, however, not observed when the animals were challenged with either histamine, serotonin or acetylcholine by aerosol. The antigen-induced expression of FcεRII/CD23 on AM was inhibited upon treatment of the rats with ketotifen or beclomethasone. In addition, oral or aerosol administration of respectively BN 50730 or BN 52021 (two structurally unrelated platelet-activating factor antagonists), inhibited the antigen-induced FcεRII/CD23 expression on AM, indicating the participation of this lipid mediator in this process.

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