Pharmacokinetics of triamcinolone acetonide following intramuscular and intra-articular administration to exercised Thoroughbred horses

Heather K Knych, M. A. Vidal, H. C. Casbeer, D. S. Mckemie

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reason for performing study: The use of triamcinolone acetonide (TA) in performance horses necessitates establishing appropriate withdrawal times prior to performance. Objectives: To describe the plasma pharmacokinetics of TA and time-related urine and synovial fluid concentrations following i.m. and intra-articular administration to exercised Thoroughbred horses. Study design: Block design. Methods: Twelve racing fit adult Thoroughbred horses received a single i.m. administration of TA (0.1mg/kg bwt). After an appropriate washout period, the same horses then received a single intra-articular TA administration (9mg) into the right antebrachiocarpal joint. Blood, urine and synovial fluid samples were collected prior to, and at various times, up to 60 days post drug administration and analysed using liquid chromatography-mass spectrometry. Plasma data were analysed using noncompartmental analysis. Results: Maximum measured plasma TA concentrations were 0.996 ± 0.391 at 13.2h and 1.27 ± 0.278ng/ml at 6.5h for i.m. and intra-articular administration, respectively. The plasma terminal elimination half-life was 11.4 ± 6.53 and 0.78 ± 1.00 days for i.m. and intra-articular administration, respectively. Following i.m. administration, TA was below the limit of detection (LOD) by Days 52 and 60 in plasma and urine, respectively. Following intra-articular administration TA was undetectable by Day 7 in plasma and Day 8 in urine. Triamcinolone acetonide was also undetectable in any of the joints sampled following i.m. administration and remained above the limit of quantitation (LOQ) for 21 days following intra-articular administration. Conclusions and potential relevance: This study extends previous studies describing the pharmacokinetics of TA following i.m. and intra-articular administration to the horse and suggests that plasma and urine concentrations are not a good indicator of synovial fluid concentrations. Furthermore, results of this study supports an extended withdrawal time for TA given i.m. The Summary is available in Chinese - see Supporting information.

Original languageEnglish (US)
Pages (from-to)715-720
Number of pages6
JournalEquine Veterinary Journal
Volume45
Issue number6
DOIs
StatePublished - Nov 2013

Fingerprint

triamcinolone
Triamcinolone Acetonide
pharmacokinetics
Horses
Pharmacokinetics
Joints
horses
urine
synovial fluid
Urine
Synovial Fluid
joints (animal)
Liquid Chromatography
liquid chromatography
half life
Half-Life
Limit of Detection
Mass Spectrometry
detection limit
experimental design

Keywords

  • Corticosteroid
  • Horse
  • Pharmacokinetics
  • Syonvial fluid
  • Thoroughbred
  • Triamacinolone acetonide (TA)

ASJC Scopus subject areas

  • Equine

Cite this

Pharmacokinetics of triamcinolone acetonide following intramuscular and intra-articular administration to exercised Thoroughbred horses. / Knych, Heather K; Vidal, M. A.; Casbeer, H. C.; Mckemie, D. S.

In: Equine Veterinary Journal, Vol. 45, No. 6, 11.2013, p. 715-720.

Research output: Contribution to journalArticle

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abstract = "Reason for performing study: The use of triamcinolone acetonide (TA) in performance horses necessitates establishing appropriate withdrawal times prior to performance. Objectives: To describe the plasma pharmacokinetics of TA and time-related urine and synovial fluid concentrations following i.m. and intra-articular administration to exercised Thoroughbred horses. Study design: Block design. Methods: Twelve racing fit adult Thoroughbred horses received a single i.m. administration of TA (0.1mg/kg bwt). After an appropriate washout period, the same horses then received a single intra-articular TA administration (9mg) into the right antebrachiocarpal joint. Blood, urine and synovial fluid samples were collected prior to, and at various times, up to 60 days post drug administration and analysed using liquid chromatography-mass spectrometry. Plasma data were analysed using noncompartmental analysis. Results: Maximum measured plasma TA concentrations were 0.996 ± 0.391 at 13.2h and 1.27 ± 0.278ng/ml at 6.5h for i.m. and intra-articular administration, respectively. The plasma terminal elimination half-life was 11.4 ± 6.53 and 0.78 ± 1.00 days for i.m. and intra-articular administration, respectively. Following i.m. administration, TA was below the limit of detection (LOD) by Days 52 and 60 in plasma and urine, respectively. Following intra-articular administration TA was undetectable by Day 7 in plasma and Day 8 in urine. Triamcinolone acetonide was also undetectable in any of the joints sampled following i.m. administration and remained above the limit of quantitation (LOQ) for 21 days following intra-articular administration. Conclusions and potential relevance: This study extends previous studies describing the pharmacokinetics of TA following i.m. and intra-articular administration to the horse and suggests that plasma and urine concentrations are not a good indicator of synovial fluid concentrations. Furthermore, results of this study supports an extended withdrawal time for TA given i.m. The Summary is available in Chinese - see Supporting information.",
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AU - Vidal, M. A.

AU - Casbeer, H. C.

AU - Mckemie, D. S.

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N2 - Reason for performing study: The use of triamcinolone acetonide (TA) in performance horses necessitates establishing appropriate withdrawal times prior to performance. Objectives: To describe the plasma pharmacokinetics of TA and time-related urine and synovial fluid concentrations following i.m. and intra-articular administration to exercised Thoroughbred horses. Study design: Block design. Methods: Twelve racing fit adult Thoroughbred horses received a single i.m. administration of TA (0.1mg/kg bwt). After an appropriate washout period, the same horses then received a single intra-articular TA administration (9mg) into the right antebrachiocarpal joint. Blood, urine and synovial fluid samples were collected prior to, and at various times, up to 60 days post drug administration and analysed using liquid chromatography-mass spectrometry. Plasma data were analysed using noncompartmental analysis. Results: Maximum measured plasma TA concentrations were 0.996 ± 0.391 at 13.2h and 1.27 ± 0.278ng/ml at 6.5h for i.m. and intra-articular administration, respectively. The plasma terminal elimination half-life was 11.4 ± 6.53 and 0.78 ± 1.00 days for i.m. and intra-articular administration, respectively. Following i.m. administration, TA was below the limit of detection (LOD) by Days 52 and 60 in plasma and urine, respectively. Following intra-articular administration TA was undetectable by Day 7 in plasma and Day 8 in urine. Triamcinolone acetonide was also undetectable in any of the joints sampled following i.m. administration and remained above the limit of quantitation (LOQ) for 21 days following intra-articular administration. Conclusions and potential relevance: This study extends previous studies describing the pharmacokinetics of TA following i.m. and intra-articular administration to the horse and suggests that plasma and urine concentrations are not a good indicator of synovial fluid concentrations. Furthermore, results of this study supports an extended withdrawal time for TA given i.m. The Summary is available in Chinese - see Supporting information.

AB - Reason for performing study: The use of triamcinolone acetonide (TA) in performance horses necessitates establishing appropriate withdrawal times prior to performance. Objectives: To describe the plasma pharmacokinetics of TA and time-related urine and synovial fluid concentrations following i.m. and intra-articular administration to exercised Thoroughbred horses. Study design: Block design. Methods: Twelve racing fit adult Thoroughbred horses received a single i.m. administration of TA (0.1mg/kg bwt). After an appropriate washout period, the same horses then received a single intra-articular TA administration (9mg) into the right antebrachiocarpal joint. Blood, urine and synovial fluid samples were collected prior to, and at various times, up to 60 days post drug administration and analysed using liquid chromatography-mass spectrometry. Plasma data were analysed using noncompartmental analysis. Results: Maximum measured plasma TA concentrations were 0.996 ± 0.391 at 13.2h and 1.27 ± 0.278ng/ml at 6.5h for i.m. and intra-articular administration, respectively. The plasma terminal elimination half-life was 11.4 ± 6.53 and 0.78 ± 1.00 days for i.m. and intra-articular administration, respectively. Following i.m. administration, TA was below the limit of detection (LOD) by Days 52 and 60 in plasma and urine, respectively. Following intra-articular administration TA was undetectable by Day 7 in plasma and Day 8 in urine. Triamcinolone acetonide was also undetectable in any of the joints sampled following i.m. administration and remained above the limit of quantitation (LOQ) for 21 days following intra-articular administration. Conclusions and potential relevance: This study extends previous studies describing the pharmacokinetics of TA following i.m. and intra-articular administration to the horse and suggests that plasma and urine concentrations are not a good indicator of synovial fluid concentrations. Furthermore, results of this study supports an extended withdrawal time for TA given i.m. The Summary is available in Chinese - see Supporting information.

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