Pharmacokinetics of different dosage of astragalus membranaceus of buyang huanwu decoction in rats with cerebral ischemic injury by microdialysis combined with LC/MS

Shengxin Wang, Xiangli Yan, Haozhen Zheng Jianye Yu, Aiming Yu, Xiao Shen, Lisheng Wang

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction (BYHWD) with different dosages of Astragalus (Yiqi groups and Huoxue groups ) applicating in rats with middle cerebral artery occlusion (MCAO). Replicating the animal model of cerebral ischemia-reperfitsion in rats, establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus (3.09, 6.17, 12.34 g/Kg) and Huoxue goups (2.32 g/Kg) when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration. The pharmacokinetics of formono etin nd paeoniflorin in the different dosage groups of BYHWD met the one-compartment model, and the t1/2of formonoretin and oaeoni florin in the low-dose Yiqi and Huoxue groups were (88.43±3.82, 69.18±0.11) min, MRT were (138.56±4.83, 113 62+2.42) min, and AUCo-twere (28 488.35±4800.32, 140 614.80±23 954.05) ng/mL-min; The t1/2of formononetir and paeoniflorin in the middle-dose Yiqi group and Huoxue group were (82.16±1.78, 67.08±3.69) min, and MRT we e (2 7. 5.70, 116.58±4.13), AUC0-twere (48 619.25±6745.75, 159 026.00±15 003.33) ng/mL-min; The t1/2of formoronetin nd paeoniflorin in the high-dose Yiqi and Huoxue groups were (80.29±1.12,69.69±0.87) min, and MRT was (128.70±1.45) min, AUC0-twere (109 942.90±13 101.83, 189 417.90±22 311.00) ng/mL-min. The concentration rate of fomoneti was decreased with increase of Astragalus dose. However, no significant difference between these two varibles wa. found during experiments. Furthermore, the experiments showed that the increasing dose of astragalus would affer th abarma behavior of paeoniflorin in the Huoxue groups. More specifically, the result showed that paemriflorin can b metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In way the erfect of paeoniflorin can be lasted for longer time in the body and brain.

Original languageEnglish (US)
Pages (from-to)90-101
Number of pages12
JournalJournal of Chinese Pharmaceutical Sciences
Volume29
Issue number2
DOIs
StatePublished - Feb 29 2020
Externally publishedYes

Keywords

  • Bu Yang Huan Wu Tag
  • Cerebral ischemia-reperfusion
  • Fharma bkinetics
  • Microdialysis

ASJC Scopus subject areas

  • Pharmaceutical Science

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