Pharmacokinetics of ceftiofur crystalline-free acid following subcutaneous administration of a single dose to sheep

Sarai Rivera-Garcia, John A Angelos, Joan D Rowe, Barbara A Byrne, Scott E. Wetzlich, Dana B. Van Liew, Lisa A Tell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective -To determine the pharmacokinetics of ceftiofur crystalline-free acid (CCFA) following SC administration of a single dose to sheep. Animals -9 healthy adult female Suffolk-crossbred sheep. Procedures -Each sheep was administered 6.6 mg of CCFA/kg, SC, in the cervical region once. Serial blood samples were collected at predetermined intervals for 14 days. Serum concentration of ceftiofur free-acid equivalents (CFAE) was determined by high-performance liquid chromatography. Pharmacokinetic parameters were determined by compartmental and noncompartmental methods. Results -Pharmacokinetics for CCFA following SC administration in sheep was best described with a 1-compartment model. Mean ± SD area under the concentration-time curve from time 0 to infinity, peak serum concentration, and time to peak serum concentration were 206.6 ± 24.8 μ•h/mL, 2.4 ± 0.5 μg/mL, and 23.1 ± 10.1 h, respectively. Serum CFAE concentrations ≥ 1 μg/mL (the target serum CFAE concentration for treatment of disease caused by Mannheimia haemolytica and Pasteurella multocida) were maintained for 2.6 to 4.9 days. No significant adverse reactions to CCFA administration were observed. Conclusions and Clinical Relevance -Results indicated that adequate therapeutic serum concentrations of CFAE for treatment of disease caused by M haemolytica and P multocida were achieved in sheep following SC administration of a single dose (6.6 mg/kg) of CCFA. Thus, CCFA might be useful for the treatment of common respiratory tract pathogens in sheep.

Original languageEnglish (US)
Pages (from-to)290-295
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume75
Issue number3
DOIs
StatePublished - Mar 2014

Fingerprint

ceftiofur
subcutaneous injection
pharmacokinetics
Sheep
Pharmacokinetics
sheep
blood serum
Acids
acids
dosage
Serum
Mannheimia haemolytica
Suffolk (sheep breed)
Pasteurella multocida
Therapeutics
respiratory system
Respiratory System
crossbreds
high performance liquid chromatography
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pharmacokinetics of ceftiofur crystalline-free acid following subcutaneous administration of a single dose to sheep. / Rivera-Garcia, Sarai; Angelos, John A; Rowe, Joan D; Byrne, Barbara A; Wetzlich, Scott E.; Van Liew, Dana B.; Tell, Lisa A.

In: American Journal of Veterinary Research, Vol. 75, No. 3, 03.2014, p. 290-295.

Research output: Contribution to journalArticle

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abstract = "Objective -To determine the pharmacokinetics of ceftiofur crystalline-free acid (CCFA) following SC administration of a single dose to sheep. Animals -9 healthy adult female Suffolk-crossbred sheep. Procedures -Each sheep was administered 6.6 mg of CCFA/kg, SC, in the cervical region once. Serial blood samples were collected at predetermined intervals for 14 days. Serum concentration of ceftiofur free-acid equivalents (CFAE) was determined by high-performance liquid chromatography. Pharmacokinetic parameters were determined by compartmental and noncompartmental methods. Results -Pharmacokinetics for CCFA following SC administration in sheep was best described with a 1-compartment model. Mean ± SD area under the concentration-time curve from time 0 to infinity, peak serum concentration, and time to peak serum concentration were 206.6 ± 24.8 μ•h/mL, 2.4 ± 0.5 μg/mL, and 23.1 ± 10.1 h, respectively. Serum CFAE concentrations ≥ 1 μg/mL (the target serum CFAE concentration for treatment of disease caused by Mannheimia haemolytica and Pasteurella multocida) were maintained for 2.6 to 4.9 days. No significant adverse reactions to CCFA administration were observed. Conclusions and Clinical Relevance -Results indicated that adequate therapeutic serum concentrations of CFAE for treatment of disease caused by M haemolytica and P multocida were achieved in sheep following SC administration of a single dose (6.6 mg/kg) of CCFA. Thus, CCFA might be useful for the treatment of common respiratory tract pathogens in sheep.",
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