Pharmacokinetic studies of the recombinant juvenile hormone esterase in Manduca sexta

Reiji Ichinose, Shizuo G. Kamita, Susumu Maeda, Bruce D. Hammock

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Catalytically active juvenile hormone esterase (JHE) was expressed at a high level by Spodoptera frugiperda cells infected with recombinant Autographa californica nuclear polyhedrosis virus carrying the JHE gene cloned from the tobacco budworm, Heliothis virescens. JHE was partially purified from the culture medium by DEAE ion-exchange chromatography to a specific activity of 3000-5200 nmol juvenile hormone III metabolized per minute per milligram of protein after viral particles were inactivated by Triton X-100 following ultracentrifugation. Pharmacokinetic studies showed that catalytic activity was cleared with first-order kinetics after the purified enzyme was injected into larval hemolymph of the tobacco hornworm, Manduca sexta and H. virescens. The half-life of JHE was approximately 1.2 hr in both species. Experiments were undertaken to elucidate the mechanism of clearance from M. sexta larvae. Persistence of catalytic activity in hemolymph in vitro and Western blot analysis suggested an uptake process of JHE by tissue(s), rather than degradation in the plasma. Injection of a high dose of JHE resulted in an increased half-life and showed that the clearance process was saturable. The clearance rate was decreased by coinjection of a high dose of JHE inactivated by an irreversible inhibitor, paraoxon. Coinjection of an equal amount of bovine serum albumin did not influence the clearance rate of JHE. These results suggest that a specific process is involved in JHE clearance from hemolymph. N-linked oligosaccharides of JHE apparently were not important in the clearance of JHE from hemolymph.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalPesticide Biochemistry and Physiology
Volume42
Issue number1
DOIs
StatePublished - 1992

Fingerprint

juvenile hormone esterase
Manduca
Pharmacokinetics
Manduca sexta
pharmacokinetics
Hemolymph
hemolymph
Heliothis virescens
Tobacco
catalytic activity
half life
Half-Life
Catalyst activity
Autographa californica multiple nucleopolyhedrovirus
Paraoxon
paraoxon
Nucleopolyhedrovirus
Spodoptera
ultracentrifugation
Spodoptera frugiperda

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biochemistry
  • Physiology

Cite this

Pharmacokinetic studies of the recombinant juvenile hormone esterase in Manduca sexta. / Ichinose, Reiji; Kamita, Shizuo G.; Maeda, Susumu; Hammock, Bruce D.

In: Pesticide Biochemistry and Physiology, Vol. 42, No. 1, 1992, p. 13-23.

Research output: Contribution to journalArticle

Ichinose, Reiji ; Kamita, Shizuo G. ; Maeda, Susumu ; Hammock, Bruce D. / Pharmacokinetic studies of the recombinant juvenile hormone esterase in Manduca sexta. In: Pesticide Biochemistry and Physiology. 1992 ; Vol. 42, No. 1. pp. 13-23.
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