Phage-displayed peptide that mimics aflatoxins and its application in immunoassay

Yanru Wang, Hong Wang, Peiwu Li, Qi Zhang, Hee Joo Kim, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.

Original languageEnglish (US)
Pages (from-to)2426-2433
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number10
DOIs
StatePublished - Mar 13 2013

Keywords

  • aflatoxin
  • matrix effect
  • mimotope
  • phage peptide library
  • phage-displayed peptide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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    Wang, Y., Wang, H., Li, P., Zhang, Q., Kim, H. J., Gee, S. J., & Hammock, B. D. (2013). Phage-displayed peptide that mimics aflatoxins and its application in immunoassay. Journal of Agricultural and Food Chemistry, 61(10), 2426-2433. https://doi.org/10.1021/jf4004048